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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0010
         (773 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD...   138   5e-33
At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy...    91   7e-19
At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehy...    39   0.004
At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate dehy...    37   0.013
At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate dehy...    37   0.013
At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein...    30   1.5  
At5g55120.1 68418.m06871 expressed protein strong similarity to ...    30   2.0  
At5g43870.1 68418.m05363 expressed protein                             29   2.6  
At4g26850.1 68417.m03865 expressed protein                             29   2.6  
At2g25170.1 68415.m03010 chromatin remodeling factor CHD3 (PICKL...    29   2.6  
At1g06100.1 68414.m00639 fatty acid desaturase family protein si...    29   2.6  
At4g23460.1 68417.m03381 beta-adaptin, putative strong similarit...    29   4.5  
At2g01290.1 68415.m00043 expressed protein                             28   6.0  
At1g06090.1 68414.m00638 fatty acid desaturase family protein si...    28   7.9  

>At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+]
           / GPDH strong similarity to  SP|P52425
           Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8)
           {Cuphea lanceolata}; contains Pfam profile PF01210:
           NAD-dependent glycerol-3-phosphate dehydrogenase
          Length = 400

 Score =  138 bits (333), Expect = 5e-33
 Identities = 75/170 (44%), Positives = 100/170 (58%), Gaps = 2/170 (1%)
 Frame = +1

Query: 4   KLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDIAEG 183
           KL  NVVA PD+  A KDA++L+FV PHQF+  IC  L GKI     A+SL+KG ++ + 
Sbjct: 120 KLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKGMEVKKE 179

Query: 184 GWHRSYITYYYKMPKNSLCCI-NGSNIASEVAEEKFCETTIGCRDVM-LAPLMRDIIQTD 357
           G     I+          CC+  G+NIA+E+A EKF E T+G R    +A     +  T 
Sbjct: 180 G--PCMISSLISKQLGINCCVLMGANIANEIAVEKFSEATVGYRGSREIADTWVQLFSTP 237

Query: 358 YFRXXXXXXXXXXXICGALKNIVAVGAGFVDGLGYGDNTKAAVIRLGLME 507
           YF            +CG LKN+VA+ AGFVDGL  G+NTKAA++R+GL E
Sbjct: 238 YFMVTPVHDVEGVELCGTLKNVVAIAAGFVDGLEMGNNTKAAIMRIGLRE 287



 Score = 95.1 bits (226), Expect = 5e-20
 Identities = 50/81 (61%), Positives = 56/81 (69%), Gaps = 2/81 (2%)
 Frame = +3

Query: 525 VFYPGSKLSTFFESCGVADLITTCYGGRNRRVAEAFVKT--GRSIKELEDEMLNGQKLQG 698
           + +P  K STFFESCGVAD+ITTC GGRNRRVAEAF K+   RS  ELE EML GQKLQG
Sbjct: 294 LLFPSVKDSTFFESCGVADVITTCLGGRNRRVAEAFAKSRGKRSFDELEAEMLQGQKLQG 353

Query: 699 PITAEEVNHMLANKNMENKFP 761
             TA EV  +L +      FP
Sbjct: 354 VSTAREVYEVLKHCGWLEMFP 374


>At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein low similarity to SP|Q26756
           Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
           (EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains
           Pfam profile PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 420

 Score = 91.1 bits (216), Expect = 7e-19
 Identities = 50/169 (29%), Positives = 78/169 (46%)
 Frame = +1

Query: 1   HKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDIAE 180
           HKLP NV+A  D   A  DAD  +  VP QF  +    +   + P    +SL KG ++  
Sbjct: 141 HKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFISLSKGLELNT 200

Query: 181 GGWHRSYITYYYKMPKNSLCCINGSNIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDY 360
                  I    K P+     ++G + A E+         +  +D  LA  ++ ++ + Y
Sbjct: 201 LRMMSQIIPIALKNPRQPFVALSGPSFALELMNNLPTAMVVASKDKKLANAVQQLLASSY 260

Query: 361 FRXXXXXXXXXXXICGALKNIVAVGAGFVDGLGYGDNTKAAVIRLGLME 507
            R           I GALKN++A+ AG VDG+  G+N+ AA++  G  E
Sbjct: 261 LRINTSSDVTGVEIAGALKNVLAIAAGIVDGMNLGNNSMAALVSQGCSE 309


>At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein weak similarity to
           SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
           (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
           PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 455

 Score = 38.7 bits (86), Expect = 0.004
 Identities = 43/162 (26%), Positives = 70/162 (43%), Gaps = 9/162 (5%)
 Frame = +1

Query: 28  VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIAEGGWHRS 198
           V ++ EA  DAD++I  +P      + + +    K    A   +SL KG + AE   H  
Sbjct: 141 VTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVE-AEFEPHPR 199

Query: 199 YITYYYKM------PKNSLCCINGSNIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDY 360
            +T    +      P  ++  + G NIASEV  +++    I   +    PL + + Q+ +
Sbjct: 200 IVTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGKFLRQS-H 258

Query: 361 FRXXXXXXXXXXXICGALKNIVAVGAGFVDGLGYGDNTKAAV 486
           F            + G LKN+ A+GAG V  L     T  +V
Sbjct: 259 FIVWDNSDLITHEVMGGLKNVYAIGAGMVATLTKESATSKSV 300


>At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein weak similarity to
           SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
           (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
           PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 462

 Score = 37.1 bits (82), Expect = 0.013
 Identities = 39/161 (24%), Positives = 67/161 (41%), Gaps = 8/161 (4%)
 Frame = +1

Query: 28  VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIA-EGGWH- 192
           V ++ EA  DAD+++  +P    R +   +    K        +SL KG + A E   H 
Sbjct: 149 VTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLSKGIETALEPVPHI 208

Query: 193 ---RSYITYYYKMPKNSLCCINGSNIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYF 363
                 I     +P +++  + G NIA+E+  +++    I        PL +  ++  +F
Sbjct: 209 ITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGAAKWRKPLAK-FLRQPHF 267

Query: 364 RXXXXXXXXXXXICGALKNIVAVGAGFVDGLGYGDNTKAAV 486
                       + G LKN+ A+GAG V  L     T  +V
Sbjct: 268 IVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSV 308


>At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein weak similarity to
           SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
           (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
           PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 462

 Score = 37.1 bits (82), Expect = 0.013
 Identities = 39/161 (24%), Positives = 67/161 (41%), Gaps = 8/161 (4%)
 Frame = +1

Query: 28  VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIA-EGGWH- 192
           V ++ EA  DAD+++  +P    R +   +    K        +SL KG + A E   H 
Sbjct: 149 VTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLSKGIETALEPVPHI 208

Query: 193 ---RSYITYYYKMPKNSLCCINGSNIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYF 363
                 I     +P +++  + G NIA+E+  +++    I        PL +  ++  +F
Sbjct: 209 ITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGAAKWRKPLAK-FLRQPHF 267

Query: 364 RXXXXXXXXXXXICGALKNIVAVGAGFVDGLGYGDNTKAAV 486
                       + G LKN+ A+GAG V  L     T  +V
Sbjct: 268 IVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSV 308


>At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein
           contains Pfam domain PF00098: Zinc knuckle
          Length = 474

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 12/40 (30%), Positives = 17/40 (42%)
 Frame = +2

Query: 362 SGSWWWTMRTQSKYVER*RTLWQWVRVSWMASATVITQRL 481
           S  WWW     +++ ER   +  W R+ W   A    Q L
Sbjct: 151 SALWWWDQDEYNRWYERRAPIRTWERLKWNMCAKYSPQSL 190


>At5g55120.1 68418.m06871 expressed protein strong similarity to
           unknown protein (pir||T04808)
          Length = 431

 Score = 29.9 bits (64), Expect = 2.0
 Identities = 10/28 (35%), Positives = 19/28 (67%)
 Frame = -3

Query: 237 QGIFRHLVIICDIRSMPATFGYIKSLNQ 154
           +G+FR+ V  C+ + +P  +G+I  LN+
Sbjct: 78  RGLFRYDVTACETKVIPGKYGFIAQLNE 105


>At5g43870.1 68418.m05363 expressed protein 
          Length = 453

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 15/57 (26%), Positives = 27/57 (47%)
 Frame = +2

Query: 350 RQTTSGSWWWTMRTQSKYVER*RTLWQWVRVSWMASATVITQRLLSSDSVSWRDQIR 520
           +  T G  WW      K  E      +++  +W ASAT +++ +++S   S   Q+R
Sbjct: 4   KSKTIGEQWWRSNPTFKPPETPLDSMEFLSRTWSASATEVSRAVVASPPTSQPPQMR 60


>At4g26850.1 68417.m03865 expressed protein
          Length = 442

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 9/28 (32%), Positives = 19/28 (67%)
 Frame = -3

Query: 237 QGIFRHLVIICDIRSMPATFGYIKSLNQ 154
           +G+FR+ V  C+ + +P  +G++  LN+
Sbjct: 84  RGLFRYDVTACETKVIPGKYGFVAQLNE 111


>At2g25170.1 68415.m03010 chromatin remodeling factor CHD3 (PICKLE)
            identical to chromatin remodeling factor CHD3
            [Arabidopsis thaliana] GI:6478518
          Length = 1384

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 14/34 (41%), Positives = 20/34 (58%)
 Frame = +3

Query: 642  GRSIKELEDEMLNGQKLQGPITAEEVNHMLANKN 743
            G S  E++DEML+G     PIT+EE+     + N
Sbjct: 1220 GVSFIEVDDEMLDGLPKTDPITSEEIMGAAVDNN 1253


>At1g06100.1 68414.m00639 fatty acid desaturase family protein
           similar to delta 9 acyl-lipid desaturase (ADS1)
           GI:2970034 from [Arabidopsis thaliana]
          Length = 299

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
 Frame = -3

Query: 300 GRFAEFFLSNLRCNIAPINTAQGIFRHLVIICDIRSMPATFGYIKSLNQRQSSC-SWLYF 124
           GR +     +L C +AP N     FR  +I+  + ++  TF Y ++L  R      WL +
Sbjct: 34  GRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEY 93


>At4g23460.1 68417.m03381 beta-adaptin, putative strong similarity
           to SP|Q10567 Adapter-related protein complex 1 beta 1
           subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin
           [Drosophila melanogaster] GI:434902; contains Pfam
           profile: PF01602 Adaptin N terminal region
          Length = 893

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 11/33 (33%), Positives = 22/33 (66%)
 Frame = -1

Query: 329 GASITSRQPMVVSQNFSSATSDAILLPLIQHRE 231
           GAS  +  PMV+SQN S+ ++ ++L   +++ +
Sbjct: 733 GASARTMMPMVLSQNMSTGSTSSVLQVAVKNNQ 765


>At2g01290.1 68415.m00043 expressed protein
          Length = 265

 Score = 28.3 bits (60), Expect = 6.0
 Identities = 16/38 (42%), Positives = 20/38 (52%)
 Frame = +1

Query: 409 ALKNIVAVGAGFVDGLGYGDNTKAAVIRLGLMERSNSL 522
           A K +  V +G V GLG G   K AV R+G + R   L
Sbjct: 41  AYKAVEFVESGMVLGLGTGSTAKHAVDRIGELLRQGKL 78


>At1g06090.1 68414.m00638 fatty acid desaturase family protein
           similar to delta 9 acyl-lipid desaturase GB:BAA25180
           GI:2970034 (ADS1) from [Arabidopsis thaliana]
          Length = 299

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 15/51 (29%), Positives = 24/51 (47%), Gaps = 1/51 (1%)
 Frame = -3

Query: 273 NLRCNIAPINTAQGIFRHLVIICDIRSMPATFGYIKSLNQRQSSC-SWLYF 124
           +L C +AP N      R  VI+  + S+  TF Y ++L  +      WL +
Sbjct: 43  HLLCLLAPFNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEY 93


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,562,417
Number of Sequences: 28952
Number of extensions: 341339
Number of successful extensions: 953
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 922
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 948
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1726528800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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