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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0022
         (685 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g53510.1 68414.m06068 mitogen-activated protein kinase, putat...    29   2.9  
At1g31460.1 68414.m03852 expressed protein                             29   3.8  
At3g13530.1 68416.m01701 MAP3K epsilon protein kinase identical ...    28   6.6  
At3g02490.1 68416.m00237 pentatricopeptide (PPR) repeat-containi...    28   6.6  
At2g36990.1 68415.m04537 RNA polymerase sigma subunit SigF (sigF...    28   6.6  

>At1g53510.1 68414.m06068 mitogen-activated protein kinase, putative
           / MAPK, putative (MPK18) mitogen-activated protein
           kinase (MAPK)(AtMPK18), PMID:12119167
          Length = 603

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 18/55 (32%), Positives = 24/55 (43%)
 Frame = +3

Query: 294 FFQRCLPLGTVLGLGTFAAIQKGHLKPNPRFGPFPKVTLAVVVGYFLGKLSYQQA 458
           +FQ  LP    L   T  AI    L+   +FGP     +AV     +G +SY  A
Sbjct: 548 YFQPQLPKTDQLNNNTHMAIDAKLLQAQSQFGPAGAAAVAVAAHRNIGTISYSAA 602


>At1g31460.1 68414.m03852 expressed protein
          Length = 301

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = -2

Query: 528 SILPDLLVSALYSCRAMPSASQHMPAGMTTSPRSSQPL 415
           S LPD L S     +++P+A +H P   ++S    +PL
Sbjct: 42  STLPDDLESRFRRLKSLPAAPRHEPVSSSSSMNRQKPL 79


>At3g13530.1 68416.m01701 MAP3K epsilon protein kinase identical to
           MAP3K epsilon protein kinase [Arabidopsis thaliana]
           gi|3549652|emb|CAA12272
          Length = 1368

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 13/28 (46%), Positives = 18/28 (64%)
 Frame = +3

Query: 366 LKPNPRFGPFPKVTLAVVVGYFLGKLSY 449
           +KPN +FGPFP+  +AV +   L  L Y
Sbjct: 108 IKPN-KFGPFPESLVAVYIAQVLEGLVY 134


>At3g02490.1 68416.m00237 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 665

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 13/24 (54%), Positives = 17/24 (70%)
 Frame = +3

Query: 3   FYKWTELKAYPSVVSRKSVSIMLR 74
           F+KW  L+AYP  +S KS + MLR
Sbjct: 130 FFKWG-LEAYPQKLSSKSYNTMLR 152


>At2g36990.1 68415.m04537 RNA polymerase sigma subunit SigF (sigF) /
           sigma-like factor (SIG6) identical to RNA polymerase
           sigma subunit SigF [Arabidopsis thaliana] GI:7209640;
           contains Pfam profiles PF04545: Sigma-70, region 4,
           PF04539: Sigma-70 region 3, PF04542: Sigma-70 region 2;
           identical to cDNA partial mRNA for putative sigma-like
           transcription factor (sig6 gene) GI:6273429
          Length = 547

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 16/45 (35%), Positives = 27/45 (60%), Gaps = 2/45 (4%)
 Frame = -2

Query: 543 SPSNLSILPDLLVSALYSCRAMPSASQHMPAG-MTTSPR-SSQPL 415
           SPS++ +L D   + + + R + S S+H PA  ++  PR  S+PL
Sbjct: 26  SPSSVVMLHDQTTTPVVNSRHLNSLSRHFPASVLSQEPREESRPL 70


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,669,521
Number of Sequences: 28952
Number of extensions: 311204
Number of successful extensions: 783
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 764
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 783
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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