Silkworm BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0019
         (724 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g47880.1 68418.m05915 eukaryotic peptide chain release factor...   217   6e-57
At3g26618.1 68416.m03325 eukaryotic release factor 1 family prot...   215   2e-56
At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot...   207   7e-54
At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains ...    29   2.4  
At4g14480.1 68417.m02233 protein kinase family protein contains ...    29   4.1  
At2g39260.1 68415.m04821 MIF4G domain-containing protein similar...    28   5.5  
At4g02420.1 68417.m00327 lectin protein kinase, putative similar...    28   7.2  
At3g59610.1 68416.m06651 F-box family protein / jacalin lectin f...    28   7.2  
At3g03710.1 68416.m00375 polyribonucleotide nucleotidyltransfera...    27   9.5  
At1g51910.1 68414.m05851 protein kinase family protein contains ...    25   9.9  

>At5g47880.1 68418.m05915 eukaryotic peptide chain release factor
           subunit 1-1 (ERF1-1) identical to SP|Q39097 Eukaryotic
           peptide chain release factor subunit 1-1 (eRF1-1)
           (Eukaryotic release factor 1-1) (Omnipotent suppressor
           protein 1 homolog 1) (SUP1 homolog 1) {Arabidopsis
           thaliana}, eukaryotic release factor 1 homolog
           GI:1155261 from [Arabidopsis thaliana]; contains Pfam
           profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2,
           PF03465 eRF1 domain 3
          Length = 436

 Score =  217 bits (530), Expect = 6e-57
 Identities = 100/127 (78%), Positives = 114/127 (89%)
 Frame = +2

Query: 344 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASN 523
           M +++  D+N+EIWKIKKLIKSLE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GTASN
Sbjct: 1   MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 524 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 703
           IKSRVNR SVLGAITS Q RLKLY +VPPNGLV+Y GTIV E+GKEKKV IDFEPF+PIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKVTIDFEPFRPIN 120

Query: 704 TSLYLCD 724
            SLYLCD
Sbjct: 121 ASLYLCD 127


>At3g26618.1 68416.m03325 eukaryotic release factor 1 family protein
           / eRF1 family protein contains Pfam profiles: PF03463
           eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
           domain 3
          Length = 435

 Score =  215 bits (525), Expect = 2e-56
 Identities = 99/124 (79%), Positives = 112/124 (90%)
 Frame = +2

Query: 353 ESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKS 532
           +  +D+N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ++RV+KMLADE+GTASNIKS
Sbjct: 3   DQESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASNIKS 62

Query: 533 RVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSL 712
           RVNR SVL AITS Q RLKLY KVPPNGLV+Y GTIVT++GKEKKV IDFEPFKPIN SL
Sbjct: 63  RVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPINASL 122

Query: 713 YLCD 724
           YLCD
Sbjct: 123 YLCD 126


>At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein
           / eRF1 family protein contains Pfam profiles: PF03463
           eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
           domain 3
          Length = 434

 Score =  207 bits (505), Expect = 7e-54
 Identities = 99/127 (77%), Positives = 110/127 (86%)
 Frame = +2

Query: 344 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASN 523
           M+EE  AD N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GTASN
Sbjct: 1   MAEE--ADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 58

Query: 524 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 703
           IKSRVNR SVL AITS Q RLKLY KVP NGLV+Y GTIV ++GKEKKV  DFEPF+PIN
Sbjct: 59  IKSRVNRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKVTFDFEPFRPIN 118

Query: 704 TSLYLCD 724
            SLYLCD
Sbjct: 119 ASLYLCD 125


>At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains
           similarity to NAD kinase [Homo sapiens]
           gi|20070086|gb|AAM01195; contains Pfam domain, PF01513:
           ATP-NAD kinase
          Length = 985

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = +2

Query: 404 KSLEMARGNGTSMISLIIPPKDQISR 481
           KS+   RGNG S   + +PP D +SR
Sbjct: 561 KSISDNRGNGFSAAPIAVPPSDNLSR 586


>At4g14480.1 68417.m02233 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 487

 Score = 28.7 bits (61), Expect = 4.1
 Identities = 16/54 (29%), Positives = 30/54 (55%)
 Frame = +2

Query: 335 RNKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKML 496
           +N+  E    D+++ + K+K L KSLE  R   + +I  +   K++ SR  ++L
Sbjct: 407 KNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELL 460


>At2g39260.1 68415.m04821 MIF4G domain-containing protein similar to
           hUPF2 [Homo sapiens] GI:12232320; contains Pfam profile
           PF02854: MIF4G domain
          Length = 1186

 Score = 28.3 bits (60), Expect = 5.5
 Identities = 23/90 (25%), Positives = 40/90 (44%)
 Frame = +2

Query: 407 SLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRL 586
           SLE+       + +L    KD  S + +ML DEF +  + K ++N  + +  I  +    
Sbjct: 513 SLELLAYYSRMVATLASCMKDIPSMLVQMLEDEFNSLVHKKDQMNIETKIRNIRFIGELC 572

Query: 587 KLYTKVPPNGLVIYCGTIVTEEGKEKKVNI 676
           K   K+ P GLV  C     +E     +++
Sbjct: 573 KF--KIVPAGLVFSCLKACLDEFTHHNIDV 600


>At4g02420.1 68417.m00327 lectin protein kinase, putative similar to
           receptor lectin kinase 3 [Arabidopsis thaliana]
           gi|4100060|gb|AAD00733; contains protein kinase domain,
           Pfam:PF00069; contains legume lectins alpha and beta
           domains, Pfam:PF00138 and Pfam:PF00139
          Length = 669

 Score = 27.9 bits (59), Expect = 7.2
 Identities = 19/64 (29%), Positives = 30/64 (46%)
 Frame = +2

Query: 230 PFHSIRLFKVLVLCKDIILVIYTNIVFVVN*LNKKRNKMSEESSADRNVEIWKIKKLIKS 409
           P    R +K  V    ++L+ +  I+F+V  + K+R K +EE   D   E  K +   K 
Sbjct: 284 PTRVYRFYKNWVPLISLLLIPFLLIIFLVRFIMKRRRKFAEEVE-DWETEFGKNRLRFKD 342

Query: 410 LEMA 421
           L  A
Sbjct: 343 LYYA 346


>At3g59610.1 68416.m06651 F-box family protein / jacalin lectin
           family protein contains Pfam profile PF01419:
           Jacalin-like lectin domain
          Length = 521

 Score = 27.9 bits (59), Expect = 7.2
 Identities = 12/45 (26%), Positives = 25/45 (55%)
 Frame = +2

Query: 344 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQIS 478
           + +E    RN+EIW  K+ IK+ +       +++S+++P    +S
Sbjct: 251 LEQEIYNTRNIEIWVTKETIKNGDGEAVEWVNLMSVLVPEWSSLS 295


>At3g03710.1 68416.m00375 polyribonucleotide nucleotidyltransferase,
           putative similar to polynucleotide phosphorylase
           GB:AAC50039 [Pisum sativum], identical to putative
           polynucleotide phosphorylase GB:AAF00646 [Arabidopsis
           thaliana]
          Length = 922

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 20/75 (26%), Positives = 37/75 (49%)
 Frame = +2

Query: 401 IKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQH 580
           I+ L    G    + ++ +PP +    V ++  +E   A  IKS+++R     AI+S++ 
Sbjct: 302 IEVLAKKYGKPKMLDAIRLPPPELYKHVKELAGEELTKALQIKSKISRRK---AISSLEE 358

Query: 581 RLKLYTKVPPNGLVI 625
             K+ T +   G VI
Sbjct: 359 --KVLTILTEKGYVI 371


>At1g51910.1 68414.m05851 protein kinase family protein contains
           Serine/Threonine protein kinases active-site signature,
           PROSITE:PS00108
          Length = 876

 Score = 25.0 bits (52), Expect(2) = 9.9
 Identities = 9/25 (36%), Positives = 17/25 (68%)
 Frame = -2

Query: 291 ITKIMSLHNTKTLNKRILWKGSPNS 217
           ++ +M++  T  L+K+I W+G P S
Sbjct: 368 VSAMMNIKKTYGLSKKISWQGDPCS 392



 Score = 20.6 bits (41), Expect(2) = 9.9
 Identities = 9/19 (47%), Positives = 12/19 (63%)
 Frame = -2

Query: 357 DSSDILFRFLFN*LTTNTI 301
           +SSD  F F F   TT+T+
Sbjct: 325 NSSDGFFNFTFTMTTTSTL 343


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,485,531
Number of Sequences: 28952
Number of extensions: 320464
Number of successful extensions: 800
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 776
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 800
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)