SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0016
         (715 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-...   357   3e-99
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei...    82   3e-16
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei...    81   9e-16
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei...    64   7e-11
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei...    62   5e-10
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460...    52   5e-07
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460...    50   2e-06
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329...    48   5e-06
At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas...    45   6e-05
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329...    43   2e-04
At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P329...    34   0.081
At1g11660.1 68414.m01339 heat shock protein, putative strong sim...    32   0.33 
At2g35060.1 68415.m04301 potassium transporter family protein si...    30   1.3  
At4g04850.1 68417.m00707 K+ efflux antiporter, putative (KEA3) s...    30   1.8  

>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
           beta-alanine synthase, putative similar to beta-alanine
           synthase [Dictyostelium discoideum] GI:14334061;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 408

 Score =  357 bits (879), Expect = 3e-99
 Identities = 163/237 (68%), Positives = 193/237 (81%)
 Frame = +3

Query: 3   FEIKAYDFPARKEECRKPRIVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQ 182
           F+++A  F A KE+ R PR+VR+GLIQ+SIA+ T  P + Q   IF+K++ II AA    
Sbjct: 70  FDLQAASFSADKEQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAG 129

Query: 183 VNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDD 362
           VNILCLQEAW MPFAFCTRE++ WC+FAEPV  G ST FL ELA KY+MVI+SPILERD 
Sbjct: 130 VNILCLQEAWTMPFAFCTRERR-WCEFAEPV-DGESTKFLQELAKKYNMVIVSPILERDI 187

Query: 363 IHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAIN 542
            HG+ +WNTAV+I   G +IGKHRKNHIPRVGDFNESTYY EG+TGHPVFET +GK+A+N
Sbjct: 188 DHGEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETVFGKIAVN 247

Query: 543 ICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
           ICYGRHHPLNWL FG+NGAEIVFNPSATV  LSE +W +EARNAAIANSY+  +INR
Sbjct: 248 ICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINR 304


>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
           low similarity to beta-alanine synthase [Drosophila
           melanogaster] GI:14334063; contains Pfam profile
           PF00795: hydrolase, carbon-nitrogen family
          Length = 299

 Score = 82.2 bits (194), Expect = 3e-16
 Identities = 47/159 (29%), Positives = 87/159 (54%), Gaps = 1/159 (0%)
 Frame = +3

Query: 150 QKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDM 329
           ++++  A A+  NI+ +QE +   + FC  +++ +   A+P    P+   + +LA +  +
Sbjct: 31  ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89

Query: 330 VI-ISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHP 506
           VI +S   E +  H    +N+  +I+  G  +G +RK+HIP    + E  Y+  G+TG  
Sbjct: 90  VIPVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFK 145

Query: 507 VFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
           VF+TK+ K+ + IC+ +  P       + GAEI+F P+A
Sbjct: 146 VFQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTA 184


>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
           low similarity to beta-alanine synthase [Drosophila
           melanogaster] GI:14334063; contains Pfam profile
           PF00795: hydrolase, carbon-nitrogen family
          Length = 326

 Score = 80.6 bits (190), Expect = 9e-16
 Identities = 47/157 (29%), Positives = 85/157 (54%), Gaps = 1/157 (0%)
 Frame = +3

Query: 156 IISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVI 335
           ++  A A+  NI+ +QE +   + FC  +++ +   A+P    P+   + +LA +  +VI
Sbjct: 60  LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118

Query: 336 -ISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVF 512
            +S   E +  H    +N+  +I+  G  +G +RK+HIP    + E  Y+  G+TG  VF
Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVF 174

Query: 513 ETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
           +TK+ K+ + IC+ +  P       + GAEI+F P+A
Sbjct: 175 QTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTA 211


>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
           similar to Nit protein 2 [Homo sapiens] GI:9367116;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 369

 Score = 64.5 bits (150), Expect = 7e-11
 Identities = 58/209 (27%), Positives = 90/209 (43%), Gaps = 12/209 (5%)
 Frame = +3

Query: 114 ITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTG--- 284
           +T  +       +K I  AA++   ++ L E WN P++        +  +AE +  G   
Sbjct: 97  VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPYS-----NDSFPVYAEEIDAGGDA 151

Query: 285 -PSTVFLAELAVKYDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH----- 443
            PST  L+E++ +  + II   I ER    GD ++NT  V    G++  KHRK H     
Sbjct: 152 SPSTAMLSEVSKRLKITIIGGSIPERV---GDRLYNTCCVFGSDGELKAKHRKIHLFDID 208

Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
           IP    F ES     G T   + +T  G++ I ICY        +++   GA ++  P A
Sbjct: 209 IPGKITFMESKTLTAGET-PTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGA 267

Query: 624 TVSGLSEHLWAVEARNAAIANSYY--TCA 704
                    W +  R  A  N  Y  TC+
Sbjct: 268 FNMTTGPLHWELLQRARATDNQLYVATCS 296


>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
           similar to Nit protein 2 [Homo sapiens] GI:9367116;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 294

 Score = 61.7 bits (143), Expect = 5e-10
 Identities = 55/204 (26%), Positives = 87/204 (42%), Gaps = 10/204 (4%)
 Frame = +3

Query: 114 ITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTG--- 284
           +T  +       +K I  AA++   ++ L E WN P++        +  +AE +  G   
Sbjct: 97  VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPYS-----NDSFPVYAEEIDAGGDA 151

Query: 285 -PSTVFLAELAVKYDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH----- 443
            PST  L+E++ +  + II   I ER    GD ++NT  V    G++  KHRK H     
Sbjct: 152 SPSTAMLSEVSKRLKITIIGGSIPERV---GDRLYNTCCVFGSDGELKAKHRKIHLFDID 208

Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
           IP    F ES     G T   + +T  G++ I ICY        +++   GA ++  P A
Sbjct: 209 IPGKITFMESKTLTAGET-PTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGA 267

Query: 624 TVSGLSEHLWAVEARNAAIANSYY 695
                    W +  R  A  N  +
Sbjct: 268 FNMTTGPLHWELLQRARATDNQVH 291


>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
           Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 355

 Score = 51.6 bits (118), Expect = 5e-07
 Identities = 40/145 (27%), Positives = 65/145 (44%), Gaps = 1/145 (0%)
 Frame = +3

Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
           GP    LA +A KY + ++  ++ER+   G T++ T +  +  G  +GKHRK  +P    
Sbjct: 115 GPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHRKL-MPTA-- 168

Query: 462 FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHP-LNWLMFGINGAEIVFNPSATVSGL 638
                + F   +  PVF+T  GK+   IC+    P L   M+   G EI   P+A     
Sbjct: 169 LERCIWGFGDGSTIPVFDTPIGKIGAAICWENRMPSLRTAMYA-KGIEIYCAPTAD---- 223

Query: 639 SEHLWAVEARNAAIANSYYTCAINR 713
           S   W     + A+    +  + N+
Sbjct: 224 SRETWLASMTHIALEGGCFVLSANQ 248


>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
           Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 346

 Score = 50.0 bits (114), Expect = 2e-06
 Identities = 37/118 (31%), Positives = 58/118 (49%), Gaps = 1/118 (0%)
 Frame = +3

Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
           GP    LAELA K ++ ++   +E+D   G T++ TA+  +  G+ +GKHRK     +  
Sbjct: 104 GPEVERLAELAGKNNVHLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRKVMPTSL-- 158

Query: 462 FNESTYYFEGNTGH-PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVS 632
             E   + +G+    PV++T  GK+   IC+    PL        G EI   P+A  S
Sbjct: 159 --ERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYRTALYAKGIEIYCAPTADYS 214


>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
           Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 339

 Score = 48.4 bits (110), Expect = 5e-06
 Identities = 35/115 (30%), Positives = 57/115 (49%), Gaps = 1/115 (0%)
 Frame = +3

Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
           GP    LAELA K ++ ++   +E+D   G T++ TA+  +  G+ +GKHRK     +  
Sbjct: 97  GPEVEKLAELAGKNNVYLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRKLMPTSL-- 151

Query: 462 FNESTYYFEGNTGH-PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
             E   + +G+    PV++T  GK+   IC+    PL        G E+   P+A
Sbjct: 152 --ERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTA 204


>At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1
           [Mus musculus] GI:3228668; contains Pfam profile
           PF00795: hydrolase, carbon-nitrogen family
          Length = 307

 Score = 44.8 bits (101), Expect = 6e-05
 Identities = 48/197 (24%), Positives = 81/197 (41%), Gaps = 8/197 (4%)
 Frame = +3

Query: 138 FEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAV 317
           F    +++  AA     ++C  E     F+F   ++      AEP L GP       LA 
Sbjct: 54  FATCSRLVQEAALAGAKLICFPEN----FSFVGDKEGESVKIAEP-LDGPVMERYCSLAR 108

Query: 318 KYDMVI-ISPILER-DDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNEST 476
             ++ + +    ER DD H   + NT VVI++ G +   ++K H     +P    + ES+
Sbjct: 109 DSNIWLSLGGFQERFDDTH---LCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKESS 165

Query: 477 YYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGING-AEIVFNPSATVSGLSEHLW 653
           +   G     V ++  G++ + +CY    P  +        A+++  PSA      E  W
Sbjct: 166 FTVPGTKIVSV-DSPVGRLGLTVCYDLRFPKIYQQLRFEQKAQVLLVPSAFTKVTGEAHW 224

Query: 654 AVEARNAAIANSYYTCA 704
            +  R  AI    Y  A
Sbjct: 225 EILLRARAIETQCYVIA 241


>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
           Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 346

 Score = 42.7 bits (96), Expect = 2e-04
 Identities = 31/115 (26%), Positives = 55/115 (47%), Gaps = 1/115 (0%)
 Frame = +3

Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
           GP    LA++A K  + ++   +E++   G T++ T +  +  G+ +GKHRK     +  
Sbjct: 104 GPEVARLADVARKNHVYLVMGAIEKE---GYTLYCTVLFFSPQGQFLGKHRKLMPTSL-- 158

Query: 462 FNESTYYFEGNTGH-PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
             E   + +G+    PV++T  GK+   IC+    PL        G E+   P+A
Sbjct: 159 --ERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTA 211


>At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P32961
           Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 224

 Score = 34.3 bits (75), Expect = 0.081
 Identities = 24/86 (27%), Positives = 40/86 (46%), Gaps = 1/86 (1%)
 Frame = +3

Query: 369 GDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGH-PVFETKYGKVAINI 545
           G T++ T +  +  G+ +GKHRK     +    E   + +G+    PV++T  GK+   I
Sbjct: 8   GYTLYCTVLFFSPQGQFLGKHRKLMPTSL----ERCIWGQGDGSTIPVYDTPIGKLGAAI 63

Query: 546 CYGRHHPLNWLMFGINGAEIVFNPSA 623
           C+    PL        G E+   P+A
Sbjct: 64  CWENRMPLYRTALYAKGIELYCAPTA 89


>At1g11660.1 68414.m01339 heat shock protein, putative strong
           similarity to gb|Z70314 heat-shock protein from
           Arabidopsis thaliana and is a member of the PF|00012
           Hsp70 protein family
          Length = 773

 Score = 32.3 bits (70), Expect = 0.33
 Identities = 19/55 (34%), Positives = 33/55 (60%)
 Frame = +3

Query: 81  QHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREK 245
           +++I + T+     +  A  EKV+K++SA A  Q+NI CL E  ++  +F  RE+
Sbjct: 250 KYNIDVYTNTKACVRLRASCEKVKKVLSANAEAQLNIECLMEEKDVR-SFIKREE 303


>At2g35060.1 68415.m04301 potassium transporter family protein
           similar to HAK2 [Hordeum vulgare] GI:7108599, potassium
           transporter HAK2p [Mesembryanthemum crystallinum]
           gi|14091471|gb|AAK53759; KUP/HAK/KT Transporter family
           member, PMID:11500563; contains Pfam profile PF02705: K+
           potassium transporter
          Length = 792

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 15/39 (38%), Positives = 20/39 (51%)
 Frame = +1

Query: 142 KRFKRSYLLQLQSKLIFFVYKKLGTCPLHFVQEKSNHGV 258
           K+F    LLQL  + +  VY  LGT PL+       HG+
Sbjct: 50  KKFSALLLLQLSFQSLGVVYGDLGTSPLYVFYNTFPHGI 88


>At4g04850.1 68417.m00707 K+ efflux antiporter, putative (KEA3)
           similar to A. thaliana K+ antiporter KEA1, GenBank
           accession number AF003382; Monovalent cation:proton
           antiporter family 2 (CPA2 family) member, PMID:11500563
          Length = 488

 Score = 29.9 bits (64), Expect = 1.8
 Identities = 15/44 (34%), Positives = 27/44 (61%)
 Frame = +3

Query: 294 VFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIG 425
           + L ++AV   +++I P+LE  DI G++IW   ++  E  K +G
Sbjct: 121 LLLQDIAV-VPLLVILPVLESQDIGGESIW--PMLAKESAKALG 161


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,432,509
Number of Sequences: 28952
Number of extensions: 325329
Number of successful extensions: 666
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 639
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 651
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -