Silkworm BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0079
         (650 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g46630.2 68418.m05740 clathrin adaptor complexes medium subun...    77   1e-14
At5g46630.1 68418.m05741 clathrin adaptor complexes medium subun...    77   1e-14
At1g60780.1 68414.m06842 clathrin adaptor complexes medium subun...    44   1e-04
At1g10730.1 68414.m01223 clathrin adaptor complexes medium subun...    34   0.071
At1g72700.1 68414.m08407 haloacid dehalogenase-like hydrolase fa...    31   0.66 
At3g44630.3 68416.m04798 disease resistance protein RPP1-WsB-lik...    29   2.0  
At3g44630.2 68416.m04800 disease resistance protein RPP1-WsB-lik...    29   2.0  
At3g44630.1 68416.m04799 disease resistance protein RPP1-WsB-lik...    29   2.0  
At5g33806.1 68418.m04019 hypothetical protein                          28   6.2  
At4g10400.1 68417.m01707 F-box family protein contains F-box dom...    28   6.2  
At3g55610.1 68416.m06177 delta 1-pyrroline-5-carboxylate synthet...    27   8.2  

>At5g46630.2 68418.m05740 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 441

 Score = 76.6 bits (180), Expect = 1e-14
 Identities = 40/82 (48%), Positives = 50/82 (60%), Gaps = 2/82 (2%)
 Frame = +2

Query: 20  LVKILTPLNTSGVQLICLKGKAKYKASENAIVWKIKRMAGMKETQLSAEIELLET-DTKK 196
           +VKI  P  T+        G+AKY  S + +VWKI++  G  E+ LSAEIEL+ T   KK
Sbjct: 326 VVKIPVPKQTAKTNFQVTTGRAKYNPSIDCLVWKIRKFPGQTESTLSAEIELISTMGEKK 385

Query: 197 KWTRPPISMGFEVP-FAPSGSR 259
            WTRPPI M F+VP F  SG R
Sbjct: 386 SWTRPPIQMEFQVPMFTASGLR 407


>At5g46630.1 68418.m05741 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 438

 Score = 76.6 bits (180), Expect = 1e-14
 Identities = 40/82 (48%), Positives = 50/82 (60%), Gaps = 2/82 (2%)
 Frame = +2

Query: 20  LVKILTPLNTSGVQLICLKGKAKYKASENAIVWKIKRMAGMKETQLSAEIELLET-DTKK 196
           +VKI  P  T+        G+AKY  S + +VWKI++  G  E+ LSAEIEL+ T   KK
Sbjct: 326 VVKIPVPKQTAKTNFQVTTGRAKYNPSIDCLVWKIRKFPGQTESTLSAEIELISTMGEKK 385

Query: 197 KWTRPPISMGFEVP-FAPSGSR 259
            WTRPPI M F+VP F  SG R
Sbjct: 386 SWTRPPIQMEFQVPMFTASGLR 407



 Score = 39.5 bits (88), Expect = 0.002
 Identities = 17/37 (45%), Positives = 27/37 (72%)
 Frame = +1

Query: 253 IKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGLYETRC 363
           ++VR+LKV+E     S ++ ++WVRYI ++G YE RC
Sbjct: 406 LRVRFLKVWEK----SGYNTVEWVRYITKAGSYEIRC 438


>At1g60780.1 68414.m06842 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 428

 Score = 43.6 bits (98), Expect = 1e-04
 Identities = 23/75 (30%), Positives = 37/75 (49%), Gaps = 2/75 (2%)
 Frame = +2

Query: 23  VKILTPLNTSGVQLICLKGKAKYKASENAIVWKIKRMAGMKETQLSAEIEL--LETDTKK 196
           +++  P + S   +    G A Y   ++A+VWKIK   G KE  L AE  L  +  +   
Sbjct: 315 IELPVPTDASNPTVRTSLGSASYAPEKDALVWKIKSFPGNKEYMLRAEFHLPSITAEEAT 374

Query: 197 KWTRPPISMGFEVPF 241
              + PI + FE+P+
Sbjct: 375 PERKAPIRVKFEIPY 389



 Score = 33.9 bits (74), Expect = 0.094
 Identities = 17/36 (47%), Positives = 22/36 (61%)
 Frame = +1

Query: 253 IKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGLYETR 360
           I+VRYLK+ E     S +  + WVRYI  +G YE R
Sbjct: 395 IQVRYLKIIEK----SGYQALPWVRYITMAGEYELR 426


>At1g10730.1 68414.m01223 clathrin adaptor complexes medium subunit
           family protein contains Pfam profile: PF00928 adaptor
           complexes medium subunit family
          Length = 428

 Score = 34.3 bits (75), Expect = 0.071
 Identities = 19/36 (52%), Positives = 22/36 (61%)
 Frame = +1

Query: 253 IKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGLYETR 360
           I+VRYLK+ E K  Y  H    WVRYI  +G YE R
Sbjct: 395 IQVRYLKIIE-KSGYQAHP---WVRYITMAGEYELR 426


>At1g72700.1 68414.m08407 haloacid dehalogenase-like hydrolase family
            protein similar to Potential phospholipid-transporting
            ATPase (EC 3.6.3.1) from Homo sapiens [SP|Q9Y2Q0,
            SP|O43520]; contains InterPro accession IPR005834:
            Haloacid dehalogenase-like hydrolase
          Length = 1228

 Score = 31.1 bits (67), Expect = 0.66
 Identities = 20/67 (29%), Positives = 36/67 (53%), Gaps = 2/67 (2%)
 Frame = +1

Query: 193  EEVDAPAHLHGVRSSLCTLRIKVRYLK--VFEPKLNYSDHDVIKWVRYIGRSGLYETRC* 366
            +E+ APA ++ + + L T+   + Y+    F+  LN  DH +I+ ++Y GR  + + R  
Sbjct: 1119 DEILAPAPIYWMATLLVTVAAVLPYVAHIAFQRFLNPLDHHIIQEIKYYGRD-IEDARLW 1177

Query: 367  TTRRRLA 387
            T  R  A
Sbjct: 1178 TRERTKA 1184


>At3g44630.3 68416.m04798 disease resistance protein RPP1-WsB-like
           (TIR-NBS-LRR class), putative domain signature
           TIR-NBS-LRR exists, suggestive of a disease resistance
           protein., closest Col-0 homolog to RPP1-WsB
          Length = 1240

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 16/63 (25%), Positives = 27/63 (42%), Gaps = 2/63 (3%)
 Frame = +2

Query: 26  KILTPLNTSGVQLICLKGKAKYKASE--NAIVWKIKRMAGMKETQLSAEIELLETDTKKK 199
           K+  P N    Q+ C+    + +  E  + I W++K +AG     L      L   +K +
Sbjct: 424 KVEYPSNDEAFQIFCMNAFGQKQPHEGFDEIAWEVKALAGKLPLGLKVLGSALRGKSKPE 483

Query: 200 WTR 208
           W R
Sbjct: 484 WER 486


>At3g44630.2 68416.m04800 disease resistance protein RPP1-WsB-like
           (TIR-NBS-LRR class), putative domain signature
           TIR-NBS-LRR exists, suggestive of a disease resistance
           protein., closest Col-0 homolog to RPP1-WsB
          Length = 1214

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 16/63 (25%), Positives = 27/63 (42%), Gaps = 2/63 (3%)
 Frame = +2

Query: 26  KILTPLNTSGVQLICLKGKAKYKASE--NAIVWKIKRMAGMKETQLSAEIELLETDTKKK 199
           K+  P N    Q+ C+    + +  E  + I W++K +AG     L      L   +K +
Sbjct: 424 KVEYPSNDEAFQIFCMNAFGQKQPHEGFDEIAWEVKALAGKLPLGLKVLGSALRGKSKPE 483

Query: 200 WTR 208
           W R
Sbjct: 484 WER 486


>At3g44630.1 68416.m04799 disease resistance protein RPP1-WsB-like
           (TIR-NBS-LRR class), putative domain signature
           TIR-NBS-LRR exists, suggestive of a disease resistance
           protein., closest Col-0 homolog to RPP1-WsB
          Length = 1214

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 16/63 (25%), Positives = 27/63 (42%), Gaps = 2/63 (3%)
 Frame = +2

Query: 26  KILTPLNTSGVQLICLKGKAKYKASE--NAIVWKIKRMAGMKETQLSAEIELLETDTKKK 199
           K+  P N    Q+ C+    + +  E  + I W++K +AG     L      L   +K +
Sbjct: 424 KVEYPSNDEAFQIFCMNAFGQKQPHEGFDEIAWEVKALAGKLPLGLKVLGSALRGKSKPE 483

Query: 200 WTR 208
           W R
Sbjct: 484 WER 486


>At5g33806.1 68418.m04019 hypothetical protein
          Length = 336

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 14/40 (35%), Positives = 19/40 (47%)
 Frame = +3

Query: 186 TPRRSGRARPSPWGSKFPLHPPDQGSLPEGVRTEAELLGP 305
           TPRR GR+  SP   + P     + S  +G     EL+ P
Sbjct: 147 TPRREGRSGRSPRRDRSPCCDSPRPSSRDGTNQRLELIAP 186


>At4g10400.1 68417.m01707 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 409

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 13/42 (30%), Positives = 20/42 (47%)
 Frame = +2

Query: 92  KASENAIVWKIKRMAGMKETQLSAEIELLETDTKKKWTRPPI 217
           K S N +VW +K    ++E  L +E +  E      W +P I
Sbjct: 295 KYSSNLLVWFLKNSPNLRELMLVSETDDHENLGMLSWNQPSI 336


>At3g55610.1 68416.m06177 delta 1-pyrroline-5-carboxylate synthetase
           B / P5CS B (P5CS2) identical to SP|P54888
          Length = 726

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 14/45 (31%), Positives = 24/45 (53%)
 Frame = +2

Query: 80  KAKYKASENAIVWKIKRMAGMKETQLSAEIELLETDTKKKWTRPP 214
           +A YK S   I W    +A +   +L A++ +L +D +  +T PP
Sbjct: 163 RAPYKDS-TGIFWDNDSLAALLSLELKADLLILLSDVEGLYTGPP 206


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,892,223
Number of Sequences: 28952
Number of extensions: 185680
Number of successful extensions: 503
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 490
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 501
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)