Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0010
(304 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 68 1e-12
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 65 7e-12
At4g18300.1 68417.m02715 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 47 3e-06
At2g34970.1 68415.m04291 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 40 2e-04
At1g77840.1 68414.m09070 eukaryotic translation initiation facto... 39 7e-04
At5g44860.1 68418.m05499 expressed protein strong similarity to ... 27 1.8
At1g03080.1 68414.m00282 kinase interacting family protein simil... 27 2.4
At4g38600.2 68417.m05463 HECT-domain-containing protein / ubiqui... 27 3.2
At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui... 27 3.2
At4g19950.1 68417.m02922 expressed protein 27 3.2
At2g23000.1 68415.m02743 serine carboxypeptidase S10 family prot... 26 4.2
At1g31130.1 68414.m03809 expressed protein 26 4.2
At1g30240.1 68414.m03699 PELP1-related contains weak similarity ... 26 4.2
At5g58670.1 68418.m07351 phosphoinositide-specific phospholipase... 25 7.4
At5g10500.1 68418.m01216 kinase interacting family protein simil... 25 7.4
At4g24510.1 68417.m03514 eceriferum protein (CER2) identical to ... 25 7.4
At2g31760.1 68415.m03878 zinc finger protein-related contains lo... 25 7.4
At1g31300.1 68414.m03830 expressed protein similar to hypothetic... 25 7.4
At3g55940.1 68416.m06216 phosphoinositide-specific phospholipase... 25 9.7
At3g52910.1 68416.m05831 expressed protein nearly identical to t... 25 9.7
At3g13100.1 68416.m01640 ABC transporter family protein similar ... 25 9.7
At2g23010.2 68415.m02745 serine carboxypeptidase S10 family prot... 25 9.7
At2g23010.1 68415.m02744 serine carboxypeptidase S10 family prot... 25 9.7
At1g77050.1 68414.m08971 DEAD/DEAH box helicase, putative simila... 25 9.7
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 67.7 bits (158), Expect = 1e-12
Identities = 28/70 (40%), Positives = 47/70 (67%)
Frame = +1
Query: 10 RAELALLTKVQEYCYENMSFMRAFSKLVVMLYKTSVLSEEVILKWYKDTSSSKGKVMFLD 189
+ EL L+ KVQ CYE+ M+ F ++V LY+ VL+E+ IL W++ ++SKG+ F+
Sbjct: 336 KLELELMYKVQMQCYEDAKLMKVFPEVVRSLYELDVLAEDTILHWFRKGTNSKGRQTFVK 395
Query: 190 QMKKFVDWLQ 219
++ FV+WL+
Sbjct: 396 SLEPFVNWLE 405
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 65.3 bits (152), Expect = 7e-12
Identities = 27/68 (39%), Positives = 45/68 (66%)
Frame = +1
Query: 16 ELALLTKVQEYCYENMSFMRAFSKLVVMLYKTSVLSEEVILKWYKDTSSSKGKVMFLDQM 195
E+ L+ KVQ CYE+ M+ F ++V LY+ VL+E+ IL WY+ ++ KG+ F+ +
Sbjct: 338 EMELMYKVQMQCYEDAKLMKVFPEVVRSLYELDVLAEDTILHWYRKGTNPKGRQTFVKGL 397
Query: 196 KKFVDWLQ 219
+ FV+WL+
Sbjct: 398 EPFVNWLE 405
>At4g18300.1 68417.m02715 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q64350
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Rattus norvegicus};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 709
Score = 46.8 bits (106), Expect = 3e-06
Identities = 20/72 (27%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
Frame = +1
Query: 7 TRAELALLTKVQEYCYENMSFMRAFSKLVVMLYKTSVLSEEVILKWYKDTSSSKG-KVMF 183
T A++ ++ K +E C E+ F+KL+ LY V+ E+ IL+W ++ + + ++
Sbjct: 628 TDAQIEVIMKFEEMCQESEELSPLFAKLLPFLYDKDVVQEDAILRWGEEKAGADDCDKVY 687
Query: 184 LDQMKKFVDWLQ 219
L++ + F+ WL+
Sbjct: 688 LNKCESFIKWLK 699
>At2g34970.1 68415.m04291 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q64350
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Rattus norvegicus};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 730
Score = 40.3 bits (90), Expect = 2e-04
Identities = 18/70 (25%), Positives = 40/70 (57%), Gaps = 2/70 (2%)
Frame = +1
Query: 16 ELALLTKVQEYCYENMSFMRA-FSKLVVMLYKTSVLSEEVILKWYKD-TSSSKGKVMFLD 189
++ ++ K +E C E+ + F++++ +LY VL E+ IL+W ++ + + ++L
Sbjct: 642 QIEVIMKFEEMCQESHKELGPLFTQILHLLYDKDVLQEDAILRWEEEKAGADEADKVYLK 701
Query: 190 QMKKFVDWLQ 219
Q F+ WL+
Sbjct: 702 QCDTFIQWLK 711
>At1g77840.1 68414.m09070 eukaryotic translation initiation factor
5, putative / eIF-5, putative similar to SP|P55876
Eukaryotic translation initiation factor 5 (eIF-5) {Zea
mays}; contains Pfam profiles PF02020:
eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
IF2B/IF5
Length = 437
Score = 38.7 bits (86), Expect = 7e-04
Identities = 20/74 (27%), Positives = 42/74 (56%), Gaps = 4/74 (5%)
Frame = +1
Query: 13 AELALLTKVQEYCYENMS--FMRAFSKLVVMLYKTSVLSEEVILKWYKD--TSSSKGKVM 180
+++ LL + +C +N + ++ + ++ LY ++ EEV+L WY+ T + K +
Sbjct: 356 SQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGLTGADKSSPV 415
Query: 181 FLDQMKKFVDWLQN 222
+ +K FV+WLQ+
Sbjct: 416 W-KNVKPFVEWLQS 428
>At5g44860.1 68418.m05499 expressed protein strong similarity to
unknown protein (gb AAC79135.1)
Length = 321
Score = 27.5 bits (58), Expect = 1.8
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = -2
Query: 240 LRLLFSILEPVYKLLHLVKEHDLALGRTSV 151
L + S+LEP+Y + + K ++L GRT++
Sbjct: 190 LASVVSVLEPIYGIAAMKKSYELLNGRTNM 219
>At1g03080.1 68414.m00282 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 1744
Score = 27.1 bits (57), Expect = 2.4
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -2
Query: 132 HLLREDARLVQHYHQLTERAHEAHVLVAVL 43
HL E+ V+ Y +RAH+AH+ + VL
Sbjct: 854 HLQDENQCRVREYQVELDRAHDAHIEIIVL 883
>At4g38600.2 68417.m05463 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1794
Score = 26.6 bits (56), Expect = 3.2
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -3
Query: 188 SRNMTLPLEELVSLYHLRI--TSSERTLVLYSITTSLLNARMKLMFS*QYSWTLVSRASS 15
S ++ L L LR+ S E+TL YS L++ L ++ W V R+ S
Sbjct: 837 SARLSSGLSALAHPLKLRLCRASGEKTLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSES 896
Query: 14 ALVP 3
AL P
Sbjct: 897 ALKP 900
>At4g38600.1 68417.m05464 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1888
Score = 26.6 bits (56), Expect = 3.2
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -3
Query: 188 SRNMTLPLEELVSLYHLRI--TSSERTLVLYSITTSLLNARMKLMFS*QYSWTLVSRASS 15
S ++ L L LR+ S E+TL YS L++ L ++ W V R+ S
Sbjct: 910 SARLSSGLSALAHPLKLRLCRASGEKTLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSES 969
Query: 14 ALVP 3
AL P
Sbjct: 970 ALKP 973
>At4g19950.1 68417.m02922 expressed protein
Length = 321
Score = 26.6 bits (56), Expect = 3.2
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = -2
Query: 240 LRLLFSILEPVYKLLHLVKEHDLALGRT 157
L + S+LEP+Y L + K ++L G+T
Sbjct: 190 LASVVSVLEPIYGLAAMKKSYELLKGKT 217
>At2g23000.1 68415.m02743 serine carboxypeptidase S10 family protein
similar to serine carboxypeptidase I precursor
(SP:P37890) [Oryza sativa]
Length = 437
Score = 26.2 bits (55), Expect = 4.2
Identities = 8/20 (40%), Positives = 15/20 (75%)
Frame = -2
Query: 111 RLVQHYHQLTERAHEAHVLV 52
RLV+ YH+ T++ + H+L+
Sbjct: 259 RLVEEYHKCTDKINTQHILI 278
>At1g31130.1 68414.m03809 expressed protein
Length = 321
Score = 26.2 bits (55), Expect = 4.2
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = -2
Query: 231 LFSILEPVYKLLHLVKEHDLALGRTSVFVPL 139
+ S+LEPVY + + K ++L G+T + + L
Sbjct: 193 VISVLEPVYGIAAMRKAYELLKGKTKMAMGL 223
>At1g30240.1 68414.m03699 PELP1-related contains weak similarity to
PELP1 [Homo sapiens] gi|21426922|gb|AAC17708
Length = 873
Score = 26.2 bits (55), Expect = 4.2
Identities = 18/47 (38%), Positives = 27/47 (57%)
Frame = -3
Query: 158 LVSLYHLRITSSERTLVLYSITTSLLNARMKLMFS*QYSWTLVSRAS 18
LVS Y + + E + LYSITT+LL + M + + Q + +V AS
Sbjct: 454 LVSSYFRKCSLPELRIKLYSITTTLLKS-MGIGMAMQLAQEVVINAS 499
>At5g58670.1 68418.m07351 phosphoinositide-specific phospholipase C
(PLC1) identical to phosphoinositide specific
phospholipase C [Arabidopsis thaliana] GI:902923
Length = 561
Score = 25.4 bits (53), Expect = 7.4
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -2
Query: 216 EPVYKLLHLVKEHDLALGRTSVFVPLEDHL 127
E + K L++VKE+ + V + LEDHL
Sbjct: 174 EDLQKCLNVVKENAFQVSAYPVVLTLEDHL 203
>At5g10500.1 68418.m01216 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 848
Score = 25.4 bits (53), Expect = 7.4
Identities = 10/40 (25%), Positives = 23/40 (57%)
Frame = -2
Query: 138 EDHLLREDARLVQHYHQLTERAHEAHVLVAVLLDLSEQGE 19
E HLL E +++++Y ++ + HE + + L ++G+
Sbjct: 555 EKHLLTEYTKVLRNYKEVKKLLHETETKLKNVNTLKDEGK 594
>At4g24510.1 68417.m03514 eceriferum protein (CER2) identical to
(CER2) [Arabidopsis thaliana] GI:1213594; contains Pfam
profile PF02458: Transferase family
Length = 421
Score = 25.4 bits (53), Expect = 7.4
Identities = 11/38 (28%), Positives = 20/38 (52%)
Frame = -3
Query: 209 STNFFIWSRNMTLPLEELVSLYHLRITSSERTLVLYSI 96
S++FF + N+T + + +Y L I + V Y+I
Sbjct: 342 SSDFFTYGANLTFVNLDEIDMYELEINGGKPDFVNYTI 379
>At2g31760.1 68415.m03878 zinc finger protein-related contains low
similarity to zinc finger proteins and Pfam PF01485: IBR
domain
Length = 514
Score = 25.4 bits (53), Expect = 7.4
Identities = 12/51 (23%), Positives = 25/51 (49%)
Frame = +1
Query: 55 ENMSFMRAFSKLVVMLYKTSVLSEEVILKWYKDTSSSKGKVMFLDQMKKFV 207
E+ ++ A +++ ++ EE ILK +D ++FL Q++ V
Sbjct: 25 ESYNYNAAVDTIILSEKSYVIIKEEEILKLQRDDIERVSTILFLSQVEAIV 75
>At1g31300.1 68414.m03830 expressed protein similar to hypothetical
protein GB:AAF24587 GI:6692122 from [Arabidopsis
thaliana]
Length = 278
Score = 25.4 bits (53), Expect = 7.4
Identities = 11/41 (26%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = +1
Query: 97 MLYKTSVLSEEVILKWYKDTSSSKGKVMF-LDQMKKFVDWL 216
M+ + + + E+ L+WY DT+ K + + ++ + F+ WL
Sbjct: 174 MVLISEITTPEINLRWYLDTAGMKKSLAYVVNGVFIFLAWL 214
>At3g55940.1 68416.m06216 phosphoinositide-specific phospholipase C,
putative similar to phosphoinositide specific
phospholipase C GI:857374 from [Arabidopsis thaliana]
Length = 584
Score = 25.0 bits (52), Expect = 9.7
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = -2
Query: 237 RLLFSILEPVYKLLHLVKEHDLALGRTSVFVPLEDHL 127
R L S +E + K L ++EH + V V LEDHL
Sbjct: 166 RTLTSPVELI-KCLRAIREHAFDVSDYPVVVTLEDHL 201
>At3g52910.1 68416.m05831 expressed protein nearly identical to
transcription activator GRL4 [Arabidopsis thaliana]
GI:21539886 (unpublished)
Length = 548
Score = 25.0 bits (52), Expect = 9.7
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = -3
Query: 203 NFFIWSRNMTLPLEELVSLYHLRITSSERTLVLYSITTSLLN 78
NFF W++ L L+ L+ Y L S + L+L I SLL+
Sbjct: 81 NFFSWAQWQELELQALIYRYMLAGASVPQELLL-PIKKSLLH 121
>At3g13100.1 68416.m01640 ABC transporter family protein similar to
ATP-binding cassette transporter MRP8 GI:18031899 from
[Arabidopsis thaliana]; contains Pfam profile: PF00005
ABC transporter
Length = 1493
Score = 25.0 bits (52), Expect = 9.7
Identities = 19/48 (39%), Positives = 23/48 (47%)
Frame = -2
Query: 282 LTRCTYLLIFFFSALRLLFSILEPVYKLLHLVKEHDLALGRTSVFVPL 139
L R ++L F FS RLL I +YK LV H L +V V L
Sbjct: 137 LLRVWWVLYFMFSCYRLLVDI--ALYKKQELVSVHLLLSDVLAVSVGL 182
>At2g23010.2 68415.m02745 serine carboxypeptidase S10 family protein
similar to serine carboxypeptidase I precursor
(SP:P37890) [Oryza sativa]
Length = 437
Score = 25.0 bits (52), Expect = 9.7
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = -2
Query: 111 RLVQHYHQLTERAHEAHVLVAVLLDLSEQ 25
+LV+ YH+ T+ + H L+A D + Q
Sbjct: 259 KLVEEYHKCTDNINSHHTLIANCDDSNTQ 287
>At2g23010.1 68415.m02744 serine carboxypeptidase S10 family protein
similar to serine carboxypeptidase I precursor
(SP:P37890) [Oryza sativa]
Length = 437
Score = 25.0 bits (52), Expect = 9.7
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = -2
Query: 111 RLVQHYHQLTERAHEAHVLVAVLLDLSEQ 25
+LV+ YH+ T+ + H L+A D + Q
Sbjct: 259 KLVEEYHKCTDNINSHHTLIANCDDSNTQ 287
>At1g77050.1 68414.m08971 DEAD/DEAH box helicase, putative similar
to RNA helicase GI:3776027 from [Arabidopsis thaliana]
Length = 513
Score = 25.0 bits (52), Expect = 9.7
Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 3/41 (7%)
Frame = -2
Query: 240 LRLLFSILEPVYK---LLHLVKEHDLALGRTSVFVPLEDHL 127
L+L F + P K LL+LV+EH + +T +FV + H+
Sbjct: 244 LKLSFLTVRPEEKYSALLYLVREHISSDQQTLIFVSTKHHV 284
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,437,280
Number of Sequences: 28952
Number of extensions: 96120
Number of successful extensions: 290
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 290
length of database: 12,070,560
effective HSP length: 70
effective length of database: 10,043,920
effective search space used: 301317600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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