Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Nnor0036
(682 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast... 206 1e-53
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri... 204 3e-53
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta... 200 7e-52
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri... 200 9e-52
At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal ident... 192 2e-49
At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron... 49 2e-06
At1g55540.1 68414.m06356 proline-rich family protein contains pr... 30 1.2
At2g36700.1 68415.m04503 pectinesterase family protein contains ... 30 1.6
At2g20780.1 68415.m02442 mannitol transporter, putative similar ... 29 3.8
At2g36960.2 68415.m04533 myb family transcription factor contain... 28 5.0
At2g36960.1 68415.m04532 myb family transcription factor contain... 28 5.0
At1g61900.2 68414.m06984 expressed protein contains similarity t... 28 6.6
At1g61900.1 68414.m06983 expressed protein contains similarity t... 28 6.6
At1g51540.1 68414.m05801 kelch repeat-containing protein contain... 28 6.6
At3g32200.1 68416.m04104 hypothetical protein 27 8.7
>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
(MDH) identical to chloroplast NAD-malate dehydrogenase
[Arabidopsis thaliana] GI:3256066; contains InterPro
entry IPR001236: Lactate/malate dehydrogenase; contains
Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
binding domain and PF02866: lactate/malate
dehydrogenase, alpha/beta C-terminal domain
Length = 403
Score = 206 bits (503), Expect = 1e-53
Identities = 100/149 (67%), Positives = 121/149 (81%)
Frame = +2
Query: 185 IGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAAIKDAD 364
IGQPL+LL+K +PLV+ L LYDIA V GVAADLSH NTP++V GP EL+ +KD +
Sbjct: 94 IGQPLSLLIKMSPLVSTLHLYDIANVK-GVAADLSHCNTPSQVRDFTGPSELADCLKDVN 152
Query: 365 VVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIASEV 544
VVVIPAGVPRKPGMTRDDLFN NA+IV+ + ++A+N P A + II+NPVNSTVPIA+EV
Sbjct: 153 VVVIPAGVPRKPGMTRDDLFNINANIVKTLVEAVAENCPNAFIHIISNPVNSTVPIAAEV 212
Query: 545 LKKAGVYDPNRVLGVTTLDVVRAATFVGE 631
LKK GVYDP ++ GVTTLDVVRA TFV +
Sbjct: 213 LKKKGVYDPKKLFGVTTLDVVRANTFVSQ 241
>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
putative similar to mitochondrial NAD-dependent malate
dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 204 bits (499), Expect = 3e-53
Identities = 102/186 (54%), Positives = 139/186 (74%), Gaps = 2/186 (1%)
Frame = +2
Query: 71 MFSRALKPAALAVQNGA--KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLAL 244
MF + +A V+ G + F++ S + K IGQPL+LL+K NPLV+ L+L
Sbjct: 1 MFRSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSL 60
Query: 245 YDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLF 424
YDIA TPGVAAD+ H+NT ++VSG+ G ++L A++ AD+V+IPAGVPRKPGMTRDDLF
Sbjct: 61 YDIAN-TPGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPGMTRDDLF 119
Query: 425 NTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDV 604
N NA IV++++++IA+ P+A+V +I+NPVNSTVPIA+E+ KKAG YD ++ GVTTLDV
Sbjct: 120 NINAGIVKNLSIAIAKYCPQALVNMISNPVNSTVPIAAEIFKKAGTYDEKKLFGVTTLDV 179
Query: 605 VRAATF 622
VRA TF
Sbjct: 180 VRARTF 185
>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
putative strong similarity to glyoxysomal malate
dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
{Arabidopsis thaliana}, SP|P37228 {Glycine max};
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 354
Score = 200 bits (488), Expect = 7e-52
Identities = 100/165 (60%), Positives = 122/165 (73%)
Frame = +2
Query: 152 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGP 331
FK IGQPLA+L+K NPLV+ L LYD+A PGV AD+SHM+T A V G G
Sbjct: 43 FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVANA-PGVTADISHMDTSAVVRGFLGQ 101
Query: 332 EELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNP 511
+L A+ D+V+IPAGVPRKPGMTRDDLFN NA IVR ++ +IA+ PKAIV II+NP
Sbjct: 102 PQLEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLSEAIAKCCPKAIVNIISNP 161
Query: 512 VNSTVPIASEVLKKAGVYDPNRVLGVTTLDVVRAATFVGEINGVD 646
VNSTVPIA+EV KKAG +DP +++GVT LDVVRA TFV E+ +D
Sbjct: 162 VNSTVPIAAEVFKKAGTFDPKKLMGVTMLDVVRANTFVAEVMSLD 206
>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
identical to mitochondrial NAD-dependent malate
dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 200 bits (487), Expect = 9e-52
Identities = 99/173 (57%), Positives = 131/173 (75%), Gaps = 1/173 (0%)
Frame = +2
Query: 122 KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNT 301
++FS+ S K IGQPLALL+K NPLV+ L+LYDIA TPGVAAD+ H+NT
Sbjct: 20 RSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIAN-TPGVAADVGHINT 78
Query: 302 PAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAP 481
++V G+ G + L+ A++ AD+V+IPAGVPRKPGMTRDDLFN NA IV+++ +IA+ P
Sbjct: 79 RSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTAIAKYCP 138
Query: 482 KAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDVVRAATF-VGEIN 637
A++ +I+NPVNSTVPIA+E+ KKAG+YD ++ GVTTLDVVRA TF G+ N
Sbjct: 139 HALINMISNPVNSTVPIAAEIFKKAGMYDEKKLFGVTTLDVVRARTFYAGKAN 191
>At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal
identical to SP|Q9ZP05; identical to cDNA microbody
NAD-dependent malate dehydrogenase GI:3929650
Length = 354
Score = 192 bits (468), Expect = 2e-49
Identities = 95/165 (57%), Positives = 118/165 (71%)
Frame = +2
Query: 152 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGP 331
FK IGQ L+LL+K NPLV+ L LYD+ PGV AD+SHM+T A V G G
Sbjct: 43 FKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNA-PGVTADVSHMDTGAVVRGFLGA 101
Query: 332 EELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNP 511
++L A+ D+V+IPAG+PRKPGMTRDDLF NA IV+ + +A+ P AIV +I+NP
Sbjct: 102 KQLEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNP 161
Query: 512 VNSTVPIASEVLKKAGVYDPNRVLGVTTLDVVRAATFVGEINGVD 646
VNSTVPIA+EV KKAG YDP ++LGVTTLDV RA TFV E+ G+D
Sbjct: 162 VNSTVPIAAEVFKKAGTYDPKKLLGVTTLDVARANTFVAEVLGLD 206
>At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong
similarity to L-lactate dehydrogenase from Lycopersicon
esculentum (GI:1620970, GI:1620972), Hordeum vulgare
(SP|P22988, SP|P22989); contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 353
Score = 49.2 bits (112), Expect = 2e-06
Identities = 42/146 (28%), Positives = 68/146 (46%), Gaps = 4/146 (2%)
Frame = +2
Query: 185 IGQPLALLLKQNPLVTRLALYDIAP-VTPGVAADLSHMNT--PAKVSGHKGPEELSAAIK 355
+G +A + L +AL D P G DL H P E++A
Sbjct: 51 VGMAIAQTILTQDLADEIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAG-- 108
Query: 356 DADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIA 535
+D+ ++ AG + PG +R +L N ++ R I +A+ +P +I+ I++NPV+ +
Sbjct: 109 -SDLCIVTAGARQNPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVD----VL 163
Query: 536 SEVLKKAGVYDPNRVLGV-TTLDVVR 610
+ V K + NRVLG T LD R
Sbjct: 164 TYVAWKLSGFPVNRVLGSGTNLDSSR 189
>At1g55540.1 68414.m06356 proline-rich family protein contains
proline rich extensin domain, INTERPRO:IPR002965
Length = 915
Score = 30.3 bits (65), Expect = 1.2
Identities = 13/54 (24%), Positives = 26/54 (48%)
Frame = +2
Query: 377 PAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPIAS 538
P P+KP + +T +++ + QN P + + I+NP + P++S
Sbjct: 518 PVSEPKKPEAQSSSILSTQSTVDSVANATKTQNEPLPVKSEISNPGTTVTPVSS 571
>At2g36700.1 68415.m04503 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 333
Score = 29.9 bits (64), Expect = 1.6
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Frame = -1
Query: 490 NSFGCILSNGQSNVTD-NGSISVE*VITGHSRFTGYSCWDDYNISIF 353
N+ GCI +NG+ + D G + V ITG +R W Y IF
Sbjct: 217 NTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIF 263
>At2g20780.1 68415.m02442 mannitol transporter, putative similar to
mannitol transporter [Apium graveolens var. dulce]
GI:12004316; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 526
Score = 28.7 bits (61), Expect = 3.8
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = +2
Query: 512 VNSTVPIASEVLKKAGVYDPNRVLGVTTLDVVRAATFV 625
+++TV + E+LK+AG+ D ++L T V F+
Sbjct: 316 IDATVYYSPEILKEAGIQDETKLLAATVAVGVTKTVFI 353
>At2g36960.2 68415.m04533 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 741
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/43 (37%), Positives = 19/43 (44%), Gaps = 1/43 (2%)
Frame = +1
Query: 202 PSTEAE-SSGDQAGFIRHSACDPRRRSRPFPHEHPSQGQRPQG 327
PS E SSGD+ + H DP PH S G+ P G
Sbjct: 635 PSQLVEASSGDEGSYNPHDDGDPMEEGPADPHTMDSPGKTPCG 677
>At2g36960.1 68415.m04532 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 743
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/43 (37%), Positives = 19/43 (44%), Gaps = 1/43 (2%)
Frame = +1
Query: 202 PSTEAE-SSGDQAGFIRHSACDPRRRSRPFPHEHPSQGQRPQG 327
PS E SSGD+ + H DP PH S G+ P G
Sbjct: 637 PSQLVEASSGDEGSYNPHDDGDPMEEGPADPHTMDSPGKTPCG 679
>At1g61900.2 68414.m06984 expressed protein contains similarity to
glutamic acid/alanine-rich protein GI:6707830 from
[Trypanosoma congolense]
Length = 413
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -1
Query: 577 AVRVVHSRLLEHFRGNRHCRVNRVC 503
A ++ SR+ E RG +C++NRVC
Sbjct: 238 ATKLDPSRVKETLRGLANCKINRVC 262
>At1g61900.1 68414.m06983 expressed protein contains similarity to
glutamic acid/alanine-rich protein GI:6707830 from
[Trypanosoma congolense]
Length = 433
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -1
Query: 577 AVRVVHSRLLEHFRGNRHCRVNRVC 503
A ++ SR+ E RG +C++NRVC
Sbjct: 238 ATKLDPSRVKETLRGLANCKINRVC 262
>At1g51540.1 68414.m05801 kelch repeat-containing protein contains
Pfam profile PF01344: Kelch motif
Length = 1036
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -1
Query: 418 VITGHSRFTGYSCWDDYNISIFDG 347
++T HS FT WD YN S+ DG
Sbjct: 314 LVTDHSSFTEKEAWDGYNKSL-DG 336
>At3g32200.1 68416.m04104 hypothetical protein
Length = 148
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +1
Query: 544 AQEGGSVRPEPRARRHHSGRSPR 612
A+ G+ RP P R+ H GRSPR
Sbjct: 35 ARSAGAFRPTPH-RKGHGGRSPR 56
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,899,205
Number of Sequences: 28952
Number of extensions: 366928
Number of successful extensions: 1009
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 980
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1004
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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