Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120040.Seq
(757 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g45310.1 68418.m05562 expressed protein 35 0.051
At5g07380.1 68418.m00845 hypothetical protein 31 1.1
At2g44400.1 68415.m05522 DC1 domain-containing protein similar ... 31 1.1
At1g09790.1 68414.m01098 phytochelatin synthetase-related contai... 29 2.5
At5g45790.1 68418.m05631 expressed protein ; expression support... 29 3.3
At5g41940.1 68418.m05106 RabGAP/TBC domain-containing protein si... 29 4.4
At4g24530.1 68417.m03516 expressed protein contains Pfam PF03138... 29 4.4
At2g25320.1 68415.m03029 meprin and TRAF homology domain-contain... 29 4.4
At1g24460.1 68414.m03081 myosin-related contains TIGRFAM TIGR016... 29 4.4
At4g21130.1 68417.m03055 transducin family protein / WD-40 repea... 28 5.8
At3g49350.1 68416.m05395 RabGAP/TBC domain-containing protein si... 28 5.8
At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A) 28 5.8
At5g15630.1 68418.m01829 phytochelatin synthetase family protein... 28 7.7
At2g22630.1 68415.m02682 MADS-box protein (AGL17) nearly identic... 28 7.7
>At5g45310.1 68418.m05562 expressed protein
Length = 352
Score = 35.1 bits (77), Expect = 0.051
Identities = 22/76 (28%), Positives = 42/76 (55%), Gaps = 5/76 (6%)
Frame = +2
Query: 248 EQHLRDRIKSK--VDEQFDQLEREYSD---KIDGFHDNIQYFKDEHYSVSCQNGSVLKSK 412
E++LR+ IK +++ D+LE E+ + KI+ +Q K+E+ + NG +SK
Sbjct: 111 EKNLREAIKEYRIMEQDLDELEDEHDEAISKIEKLEAELQELKEENLQLMEVNGKDYRSK 170
Query: 413 FAKILKSHDYTDKKSI 460
K+ S + ++ +SI
Sbjct: 171 KGKVKPSEEPSEIRSI 186
>At5g07380.1 68418.m00845 hypothetical protein
Length = 595
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +1
Query: 517 VAVCVLKPGFENGSNQVLSFEYNPIGNKVIVPFAHEINDTGL 642
+ +C + +G++Q L F Y P GN + F H IN L
Sbjct: 45 IGLCYILKDRISGNDQQLKFAYTPTGNFCLRDFHHAINSLPL 86
>At2g44400.1 68415.m05522 DC1 domain-containing protein similar to
GP|2435515|AF024504
Length = 146
Score = 30.7 bits (66), Expect = 1.1
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = -3
Query: 566 TWLLPFSNPGFNTHTAT*QCCVCRPIGANS 477
T+ +P PG + ++ QCCVCR +G+NS
Sbjct: 103 TFSMPPGGPG-SAAASSFQCCVCRGLGSNS 131
>At1g09790.1 68414.m01098 phytochelatin synthetase-related contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 454
Score = 29.5 bits (63), Expect = 2.5
Identities = 20/55 (36%), Positives = 30/55 (54%)
Frame = +1
Query: 529 VLKPGFENGSNQVLSFEYNPIGNKVIVPFAHEINDTGLYEYDVLAYVDSVEFDGK 693
VL P ++ QV SF Y K + P+ + INDTG++ + V Y D + +GK
Sbjct: 328 VLHPNLKS-VQQVFSFNY-----KSLTPYQNSINDTGMF-WGVQFYNDVLLQEGK 375
>At5g45790.1 68418.m05631 expressed protein ; expression supported
by MPSS
Length = 423
Score = 29.1 bits (62), Expect = 3.3
Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 2/37 (5%)
Frame = +3
Query: 66 CKMCVIFPVEIDVSQTVIRDCHVD--EQTRELVYINK 170
CK+ F V ID + V+R+C VD ++ ++L+ ++K
Sbjct: 129 CKITEKFKVLIDEEENVVRECEVDAVKRVKKLLLLSK 165
>At5g41940.1 68418.m05106 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 549
Score = 28.7 bits (61), Expect = 4.4
Identities = 17/49 (34%), Positives = 28/49 (57%), Gaps = 1/49 (2%)
Frame = -2
Query: 657 DVVLVKSRVVNFVSKRHNNFVTNRVVLKR*-HLVAAVLKSRLQHAYRHV 514
D VL V F+SK +NF + V ++R +V+ ++K + H YRH+
Sbjct: 380 DDVLAFWCFVGFMSKARHNFRLDEVGIRRQLSMVSKIIKFKDIHLYRHL 428
>At4g24530.1 68417.m03516 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'PsRT17-1 like protein' based on similarity to PsRT17-1
(GP:1778376) [Pisum sativum] which was based upon
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 519
Score = 28.7 bits (61), Expect = 4.4
Identities = 18/43 (41%), Positives = 20/43 (46%)
Frame = -3
Query: 407 FSTRCHFGNLPNSVHL*NTGCYREIHQFYRCIRVLVDQIVHRL 279
FS R F NLP S+ E F IR L D +VHRL
Sbjct: 226 FSHRLAFDNLPESIQRLRCKVNFEALNFVPHIRELGDALVHRL 268
>At2g25320.1 68415.m03029 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein weak similarity
to ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 1663
Score = 28.7 bits (61), Expect = 4.4
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +2
Query: 224 SYTKRYAQEQHLRDRIKSKVDEQFDQLEREYSDKIDGFHD 343
S T++ ++ + R+KS++ + D+ RE D ++ F D
Sbjct: 1376 SITQKLSESEAANTRLKSEMKAEADRFSREKKDLVEQFRD 1415
>At1g24460.1 68414.m03081 myosin-related contains TIGRFAM TIGR01612:
reticulocyte binding protein; similar to Myosin heavy
chain, non-muscle (Zipper protein) (Myosin II)
(SP:Q99323) {Drosophila melanogaster} similar to EST
gb|T76116
Length = 1730
Score = 28.7 bits (61), Expect = 4.4
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +2
Query: 248 EQHLRDRIKSKVDEQFDQLEREYSDKIDGFHDNIQYFK 361
E H I VD FD++E++ D D + N + F+
Sbjct: 617 EDHTSSDINLLVDRSFDKIEKQIRDSSDSSYGNEEIFE 654
>At4g21130.1 68417.m03055 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400); some
similarity to a group of proteins with homology to
mammalian apoptosis regulators identified in zebrafish
(PUBMED:10917738)Apaf-1(gi:7677507)
Length = 537
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/33 (45%), Positives = 19/33 (57%), Gaps = 3/33 (9%)
Frame = +3
Query: 123 DCHV---DEQTRELVYINKIMNTQLTKPVLMMF 212
DCHV D +TRE V +NK+ N Q+ V F
Sbjct: 227 DCHVHLWDIRTREHVQVNKLKNNQVKDSVHCAF 259
>At3g49350.1 68416.m05395 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 554
Score = 28.3 bits (60), Expect = 5.8
Identities = 14/43 (32%), Positives = 26/43 (60%), Gaps = 1/43 (2%)
Frame = -2
Query: 630 VNFVSKRHNNFVTNRVVLKR*-HLVAAVLKSRLQHAYRHVAVL 505
V F+ K +NF + V ++R ++V+ ++KS+ YRH+ L
Sbjct: 389 VGFMKKARHNFRLDEVGIRRQLNIVSKIIKSKDSQLYRHLEKL 431
>At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A)
Length = 135
Score = 28.3 bits (60), Expect = 5.8
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = +3
Query: 483 CPNWSTNTTLLRG 521
CP WS NT LLRG
Sbjct: 76 CPTWSENTVLLRG 88
>At5g15630.1 68418.m01829 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL4 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623); contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 431
Score = 27.9 bits (59), Expect = 7.7
Identities = 20/67 (29%), Positives = 29/67 (43%)
Frame = +1
Query: 472 KILFAPIGRQTQHCYVAVCVLKPGFENGSNQVLSFEYNPIGNKVIVPFAHEINDTGLYEY 651
KI + H + + P N + QV SF+Y P+ P+ INDTG++ Y
Sbjct: 295 KIAITNFNYRMNHTLWTLAIQHPNLNNVT-QVFSFDYKPVS-----PYG-SINDTGMF-Y 346
Query: 652 DVLAYVD 672
Y D
Sbjct: 347 GTKFYND 353
>At2g22630.1 68415.m02682 MADS-box protein (AGL17) nearly identical
to MADS-box protein AGL17 [Arabidopsis thaliana]
GI:862648
Length = 227
Score = 27.9 bits (59), Expect = 7.7
Identities = 18/61 (29%), Positives = 27/61 (44%)
Frame = -3
Query: 227 RTRNVKHHENGFCQLRVHNLVNVHQLSGLFVHMTISNNRLGHVDFYRKNYTHFAMVKLKC 48
R RN+ HHEN +V + + + T + N LGH + Y A V+L+
Sbjct: 154 RKRNLVHHENLELSRKVQRIHQENVELYKKAYGTSNTNGLGHHELVDAVYESHAQVRLQL 213
Query: 47 S 45
S
Sbjct: 214 S 214
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,443,356
Number of Sequences: 28952
Number of extensions: 316986
Number of successful extensions: 943
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 920
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 943
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1682736544
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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