Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060053.seq
(634 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 117 7e-27
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 117 7e-27
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 31 0.84
At1g76010.1 68414.m08825 expressed protein 30 1.5
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 1.9
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 29 2.6
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 29 2.6
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 29 2.6
At2g05520.1 68415.m00584 glycine-rich protein (GRP) identical to... 29 2.6
At3g22330.1 68416.m02820 DEAD box RNA helicase, putative similar... 29 3.4
At3g04640.1 68416.m00497 glycine-rich protein predicted proteins... 29 3.4
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 29 3.4
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 29 3.4
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 4.5
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 5.9
At3g12430.1 68416.m01548 expressed protein ; expression supporte... 28 5.9
At2g42200.2 68415.m05223 squamosa promoter-binding protein-like ... 27 7.8
At2g42200.1 68415.m05222 squamosa promoter-binding protein-like ... 27 7.8
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 27 7.8
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 117 bits (281), Expect = 7e-27
Identities = 49/54 (90%), Positives = 51/54 (94%)
Frame = +1
Query: 256 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 417
GHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 65 GHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 118
Score = 58.4 bits (135), Expect = 4e-09
Identities = 30/59 (50%), Positives = 37/59 (62%), Gaps = 2/59 (3%)
Frame = +2
Query: 83 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 253
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKK 63
Score = 35.1 bits (77), Expect = 0.039
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +2
Query: 509 IEKIPELPLVVADKVQEINKTKQAVIFLK 595
IE +PE+PLVV+D + + KT A+ LK
Sbjct: 150 IENVPEMPLVVSDSAEAVEKTSAAIKVLK 178
Score = 34.7 bits (76), Expect = 0.052
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +3
Query: 459 SVAATGVPALVQARGHILKRFPSFP 533
++AAT VPALV ARGH ++ P P
Sbjct: 133 AIAATAVPALVMARGHKIENVPEMP 157
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 117 bits (281), Expect = 7e-27
Identities = 49/54 (90%), Positives = 51/54 (94%)
Frame = +1
Query: 256 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 417
GHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 64 GHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 117
Score = 60.5 bits (140), Expect = 9e-10
Identities = 30/62 (48%), Positives = 41/62 (66%), Gaps = 2/62 (3%)
Frame = +2
Query: 74 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 247
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 248 KE 253
K+
Sbjct: 61 KK 62
Score = 35.1 bits (77), Expect = 0.039
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +2
Query: 509 IEKIPELPLVVADKVQEINKTKQAVIFLK 595
IE +PE+PLVV+D + + KT A+ LK
Sbjct: 149 IENVPEMPLVVSDSAEAVEKTSAAIKVLK 177
Score = 34.7 bits (76), Expect = 0.052
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +3
Query: 459 SVAATGVPALVQARGHILKRFPSFP 533
++AAT VPALV ARGH ++ P P
Sbjct: 132 AIAATAVPALVMARGHKIENVPEMP 156
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 30.7 bits (66), Expect = 0.84
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = -2
Query: 576 CLVLLISWTLSATTKGSSGIFSICV 502
CLVLL+S +A TK SGIF+ C+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFTGCL 33
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 29.9 bits (64), Expect = 1.5
Identities = 18/43 (41%), Positives = 19/43 (44%)
Frame = +1
Query: 247 QGGGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 375
QG G + G GR R R RGGG R G G F N G
Sbjct: 303 QGRGGYDGPQGRGRGRGRGRGGRGRGGG--RGGDGGFNNRSDG 343
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 1.9
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +2
Query: 98 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 244
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 29.1 bits (62), Expect = 2.6
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 388 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 269
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 29.1 bits (62), Expect = 2.6
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 388 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 269
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 29.1 bits (62), Expect = 2.6
Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 3/64 (4%)
Frame = +1
Query: 223 LEAALLREQGGGHQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 393
+ AL+R + G T AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMGAKPT-AEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 394 TKPW 405
W
Sbjct: 135 ISKW 138
>At2g05520.1 68415.m00584 glycine-rich protein (GRP) identical to
glycine-rich protein; atGRP (GI:259447) [Arabidopsis
thaliana]
Length = 145
Score = 29.1 bits (62), Expect = 2.6
Identities = 17/44 (38%), Positives = 22/44 (50%)
Frame = +1
Query: 247 QGGGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG 378
QGGG + G GR R +GGG + G G+ G+ CR G
Sbjct: 79 QGGGGRYQG---GGGRYQGGGGRYQGGGGRQGGGGSGGSYCRHG 119
>At3g22330.1 68416.m02820 DEAD box RNA helicase, putative similar to
RNA helicases GI:3775995, GI:3775987 from [Arabidopsis
thaliana]; contains Pfam profiles PF00270: DEAD/DEAH box
helicase, PF00271: Helicase conserved C-terminal domain
Length = 616
Score = 28.7 bits (61), Expect = 3.4
Identities = 16/40 (40%), Positives = 22/40 (55%)
Frame = +1
Query: 241 REQGGGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFG 360
R GGG+ S+ +G GR+ R G + RSG G+FG
Sbjct: 512 RSGGGGYGGSSGGYGGGRSGGSSNRY-SGDSDRSGFGSFG 550
>At3g04640.1 68416.m00497 glycine-rich protein predicted proteins,
Arabidopsis thaliana
Length = 159
Score = 28.7 bits (61), Expect = 3.4
Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 3/41 (7%)
Frame = +1
Query: 247 QGGGHQTSAESWGT---GRAVARIPRVRGGGTHRSGQGAFG 360
+GGG +S G+ G + IP + GGGTHRSG + G
Sbjct: 99 RGGGGSSSRGGGGSSSRGGGLRPIP-IYGGGTHRSGHHSSG 138
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 28.7 bits (61), Expect = 3.4
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +1
Query: 247 QGGGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 381
+G G + + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 28.7 bits (61), Expect = 3.4
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +1
Query: 247 QGGGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 381
+G G + + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 4.5
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +2
Query: 149 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 244
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 7.8
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +2
Query: 149 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 244
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 5.9
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -1
Query: 376 HHDTCYRRHPDRTYEYHHHGHAEF 305
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At3g12430.1 68416.m01548 expressed protein ; expression supported
by MPSS
Length = 238
Score = 27.9 bits (59), Expect = 5.9
Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = -3
Query: 152 GAWLHPAPSHSSLNTPTLKVGLPID--SFRYFSEAIPLKY 39
G W+H SH+ ++ L VG+ + Y+S + P++Y
Sbjct: 38 GQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRY 77
>At2g42200.2 68415.m05223 squamosa promoter-binding protein-like 9
(SPL9) identical to squamosa promoter binding
protein-like 9 [Arabidopsis thaliana] GI:5931673;
contains Pfam profile PF03110: SBP domain
Length = 369
Score = 27.5 bits (58), Expect = 7.8
Identities = 16/35 (45%), Positives = 19/35 (54%)
Frame = +1
Query: 244 EQGGGHQTSAESWGTGRAVARIPRVRGGGTHRSGQ 348
E GGG S+ S G RVRGGG+ +SGQ
Sbjct: 41 EDGGGGSGSSSSGGRSNR-----RVRGGGSGQSGQ 70
>At2g42200.1 68415.m05222 squamosa promoter-binding protein-like 9
(SPL9) identical to squamosa promoter binding
protein-like 9 [Arabidopsis thaliana] GI:5931673;
contains Pfam profile PF03110: SBP domain
Length = 375
Score = 27.5 bits (58), Expect = 7.8
Identities = 16/35 (45%), Positives = 19/35 (54%)
Frame = +1
Query: 244 EQGGGHQTSAESWGTGRAVARIPRVRGGGTHRSGQ 348
E GGG S+ S G RVRGGG+ +SGQ
Sbjct: 41 EDGGGGSGSSSSGGRSNR-----RVRGGGSGQSGQ 70
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.5 bits (58), Expect = 7.8
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -1
Query: 376 HHDTCYRRHPDRTYEYHHHGHAEFGRQH 293
HHD + H D +++ HH H H
Sbjct: 311 HHDHDHDHHHDHNHDHDHHHHDGHDHHH 338
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,384,170
Number of Sequences: 28952
Number of extensions: 284529
Number of successful extensions: 971
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 889
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 960
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1295224128
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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