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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021022
         (810 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g10220.1 68416.m01223 tubulin folding cofactor B identical to...    64   1e-10
At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferlin...    43   3e-04
At5g52280.1 68418.m06488 protein transport protein-related low s...    33   0.22 
At4g24700.1 68417.m03535 expressed protein                             32   0.52 
At3g22080.1 68416.m02786 meprin and TRAF homology domain-contain...    31   1.2  
At3g52640.2 68416.m05800 nicastrin-related contains weak similar...    29   3.7  
At3g52640.1 68416.m05799 nicastrin-related contains weak similar...    29   3.7  
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f...    28   6.4  
At2g23270.1 68415.m02779 expressed protein ; similar to GP|24648...    28   8.4  

>At3g10220.1 68416.m01223 tubulin folding cofactor B identical to
           tubulin folding cofactor B GI:20514259 from [Arabidopsis
           thaliana]; identical to cDNA tubulin folding cofactor B
           GI:20514258
          Length = 243

 Score = 63.7 bits (148), Expect = 1e-10
 Identities = 33/84 (39%), Positives = 47/84 (55%), Gaps = 3/84 (3%)
 Frame = +3

Query: 462 VSANWTTKVFRPQRDITEDTDSFI-IGERVWVG-GTKPGQIAYIGETQ-FAPGEWAGIVL 632
           VS N      + + +  ED  + I +G+R  V  G K G + Y+G  +   PG W GI  
Sbjct: 136 VSQNPVAAEAKTKENYMEDLCANIKVGDRCQVEPGEKRGMVKYVGRAESLGPGYWVGIQY 195

Query: 633 DEPIGKNDGSVAGVRYFQCPEKRG 704
           DEP+GK+DG V G R+F+CP  +G
Sbjct: 196 DEPLGKHDGMVKGTRFFECPRLQG 219


>At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferling
           (PFI) almost identical to tubulin folding cofactor E
           (Pfifferling; PFI) GI:20514267 from [Arabidopsis
           thaliana]; identical to cDNA tubulin folding cofactor E,
           GI:20514266
          Length = 531

 Score = 42.7 bits (96), Expect = 3e-04
 Identities = 23/57 (40%), Positives = 34/57 (59%), Gaps = 3/57 (5%)
 Frame = +3

Query: 522 DSFIIGERVWV--GGTKPGQIAYIGETQFAPGEWAGIVLDEP-IGKNDGSVAGVRYF 683
           +SFIIG+RV       + G + Y+G+ +   G W G+  D+   GK++GSV GV YF
Sbjct: 8   ESFIIGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFYF 64


>At5g52280.1 68418.m06488 protein transport protein-related low
            similarity to  SP|P25386 Intracellular protein transport
            protein USO1 {Saccharomyces cerevisiae}
          Length = 853

 Score = 33.1 bits (72), Expect = 0.22
 Identities = 21/84 (25%), Positives = 45/84 (53%), Gaps = 4/84 (4%)
 Frame = +2

Query: 254  REMIDKIQANVEEVKKKHSAILSA---PQSDENGSSTDTLRKLSDDLSR-KHLSASSTFS 421
            R+ +  ++ ++   +++ + IL A    +S ENG   + L KLSD+L+  K+ ++S    
Sbjct: 756  RKQVSNLKVDIRRKEEEMTKILDARMEARSQENGHKEENLSKLSDELAYCKNKNSSMERE 815

Query: 422  ATSMDALWEKHARRLSELDYEGLQ 493
               M+  + + + R +E++ E  Q
Sbjct: 816  LKEMEERYSEISLRFAEVEGERQQ 839


>At4g24700.1 68417.m03535 expressed protein 
          Length = 143

 Score = 31.9 bits (69), Expect = 0.52
 Identities = 25/79 (31%), Positives = 35/79 (44%)
 Frame = +2

Query: 248 RNREMIDKIQANVEEVKKKHSAILSAPQSDENGSSTDTLRKLSDDLSRKHLSASSTFSAT 427
           + RE  D+ +   EE KK+   + +A Q+  + S T     L  D  RKH       S  
Sbjct: 3   KKREAKDENEEEEEEKKKRLELMKAAAQAWLSHSQTSKHTVLEFDAQRKHAFVKGKASRF 62

Query: 428 SMDALWEKHARRLSELDYE 484
             +AL  KH    S LD+E
Sbjct: 63  KTEALSAKH--HPSFLDWE 79


>At3g22080.1 68416.m02786 meprin and TRAF homology domain-containing
           protein / MATH domain-containing protein weak similarity
           to ubiquitin-specific protease 12 [Arabidopsis thaliana]
           GI:11993471; contains Pfam profile PF00917: MATH domain
          Length = 595

 Score = 30.7 bits (66), Expect = 1.2
 Identities = 12/49 (24%), Positives = 27/49 (55%), Gaps = 2/49 (4%)
 Frame = +3

Query: 411 VRSALLRWMLFGRNTRVVSA--NWTTKVFRPQRDITEDTDSFIIGERVW 551
           V  +L +W +   N +++    +W+ K F+  ++   ++D F++G R W
Sbjct: 116 VAPSLTKWEVVSFNQKILDPKFSWSLKKFKELKEELYNSDKFLVGGRQW 164


>At3g52640.2 68416.m05800 nicastrin-related contains weak similarity
           to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
           sapiens]
          Length = 705

 Score = 29.1 bits (62), Expect = 3.7
 Identities = 11/23 (47%), Positives = 14/23 (60%)
 Frame = +3

Query: 582 YIGETQFAPGEWAGIVLDEPIGK 650
           YI  T   PG +AG++L EP  K
Sbjct: 535 YISPTNTCPGNYAGVILGEPSSK 557


>At3g52640.1 68416.m05799 nicastrin-related contains weak similarity
           to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
           sapiens]
          Length = 676

 Score = 29.1 bits (62), Expect = 3.7
 Identities = 11/23 (47%), Positives = 14/23 (60%)
 Frame = +3

Query: 582 YIGETQFAPGEWAGIVLDEPIGK 650
           YI  T   PG +AG++L EP  K
Sbjct: 506 YISPTNTCPGNYAGVILGEPSSK 528


>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
           family protein similar to SP|Q9RHV9 Lysyl-tRNA
           synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
           stearothermophilus}; contains Pfam profile: PF00152 tRNA
           synthetases class II (D, K and N)
          Length = 602

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 18/65 (27%), Positives = 31/65 (47%)
 Frame = +2

Query: 308 SAILSAPQSDENGSSTDTLRKLSDDLSRKHLSASSTFSATSMDALWEKHARRLSELDYEG 487
           S  L    +  + SS+ T  + S    R   SASS+ S +  +A+     +++ EL  +G
Sbjct: 40  SPALRCASAASSSSSSATTAETSKPSGRNRRSASSSNSTSDREAIRSIRLKKVEELRGQG 99

Query: 488 LQTTA 502
           L+  A
Sbjct: 100 LEPYA 104


>At2g23270.1 68415.m02779 expressed protein ; similar to
           GP|2464853|gnl|PID|e353149|Z99707
          Length = 86

 Score = 27.9 bits (59), Expect = 8.4
 Identities = 12/33 (36%), Positives = 18/33 (54%)
 Frame = +1

Query: 379 RPI*KTPISQQYVQRYFDGCSLGETRASSQRTG 477
           RP+  T   +  V ++FDG SLG  + S   +G
Sbjct: 26  RPLGLTKAEENLVAKFFDGLSLGAIKESGPSSG 58


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,115,523
Number of Sequences: 28952
Number of extensions: 327778
Number of successful extensions: 1082
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1034
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1078
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1843581600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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