Silkworm BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0023
         (698 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g15230.1 68415.m01737 lipase family protein similar to SP|P07...    64   9e-11
At1g18460.1 68414.m02303 lipase family protein similar to triacy...    39   0.003
At5g17110.1 68418.m02004 hypothetical protein                          31   0.56 
At3g14170.1 68416.m01791 expressed protein                             27   9.0  

>At2g15230.1 68415.m01737 lipase family protein similar to SP|P07098
           Triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
           {Homo sapiens}; contains Pfam profile PF04083:
           ab-hydrolase associated lipase region
          Length = 393

 Score = 64.1 bits (149), Expect = 9e-11
 Identities = 43/149 (28%), Positives = 75/149 (50%), Gaps = 2/149 (1%)
 Frame = +2

Query: 20  GHVRL*NAIFFICGTDEKQFNKTLLPVFLSHLGTGTSMKTILHFAQEIDAAGRFQQFDYG 199
           GH+   + +  I GT+   FN + +  +L +    +S+K I H  Q I   G F Q+DYG
Sbjct: 242 GHMDCTDFLTSITGTN-CCFNASKIEYYLDYEPHPSSVKNIRHLFQMI-RKGTFAQYDYG 299

Query: 200 PTNNMIIYNSETPPEYDLRKI--TLPIYLLYSRNDLLSSEQDVDKLYQDLETRTEIYLVP 373
              N+  Y    PPE+ L  I  +LP+++ Y   D L+   DV+    +L +  E+  + 
Sbjct: 300 YFKNLRTYGLSKPPEFILSHIPASLPMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLE 359

Query: 374 DPEFNHVDYLMANDAPRLLNDKVLQFLLA 460
           D  + H+D+++ + A   +   ++QF  A
Sbjct: 360 D--YGHIDFVLGSSAKEDVYKHMIQFFRA 386


>At1g18460.1 68414.m02303 lipase family protein similar to
           triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
           {Canis familiaris} [SP|P80035]
          Length = 701

 Score = 39.1 bits (87), Expect = 0.003
 Identities = 31/125 (24%), Positives = 58/125 (46%), Gaps = 4/125 (3%)
 Frame = +2

Query: 50  FICGTDEKQFNKTL-LPVFLSHLGTGTSMKTILHFAQEIDAAGRFQQFDYGPTN-NMIIY 223
           ++ G D   +   + LP +  +   G S +   H AQ I  +G+F+ FDYG ++ NM +Y
Sbjct: 537 YVVGGDSSNWVGVMGLPHYNMNDMPGISFRVAQHLAQ-IKHSGKFKMFDYGSSSANMDVY 595

Query: 224 NSETPPEYD--LRKITLPIYLLYSRNDLLSSEQDVDKLYQDLETRTEIYLVPDPEFNHVD 397
            S  P +       I +P+ L+  + D +     V K Y+ +          + E+ H+D
Sbjct: 596 GSPEPLDLGEFYGLIDVPVDLVAGKKDKVIRPSMVRKHYRVMRDSGVDVSYNEFEYAHLD 655

Query: 398 YLMAN 412
           +  ++
Sbjct: 656 FTFSH 660


>At5g17110.1 68418.m02004 hypothetical protein
          Length = 208

 Score = 31.5 bits (68), Expect = 0.56
 Identities = 21/82 (25%), Positives = 37/82 (45%), Gaps = 3/82 (3%)
 Frame = +2

Query: 104 LSHLGTGTSMKTILHFAQEIDAAGRFQQFDYGPTNNMIIYN--SETPPEYDLRKITLPIY 277
           +S +GT T  + +    +E D +      DYG  +    YN  +ET PE+D+       +
Sbjct: 10  VSMMGTSTKEEVVSKVDEEDDDSSSDSDMDYGSDSEWAGYNVLAETEPEWDVDSFDGHEF 69

Query: 278 LLYSR-NDLLSSEQDVDKLYQD 340
            +  R   +  S++  DK Y +
Sbjct: 70  KINPRVRQMYRSQELYDKYYNN 91


>At3g14170.1 68416.m01791 expressed protein 
          Length = 484

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 19/71 (26%), Positives = 30/71 (42%), Gaps = 1/71 (1%)
 Frame = +1

Query: 85  DAAAGVPVAPRDRHVHEDHPSL-RAGDRRRGTLPAVRLRTHQ*YDNIQFRNSTRIRSAQN 261
           DA   VPV    R +   HP +    D  +  +P+      + Y N + ++  R    +N
Sbjct: 91  DAGTPVPVLVGVRPISGRHPFVGNPKDLMQMLVPSETTPREEEYHNQKKKDGARSNIVEN 150

Query: 262 HSSHLPAVLKE 294
              H P V+KE
Sbjct: 151 IRKHQPFVIKE 161


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,865,309
Number of Sequences: 28952
Number of extensions: 345508
Number of successful extensions: 997
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 961
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 995
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)