Silkworm BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NRPG0002
         (594 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g45300.1 68416.m04891 isovaleryl-CoA-dehydrogenase (IVD) iden...    54   1e-07
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ...    31   0.58 
At5g60090.1 68418.m07534 protein kinase family protein contains ...    28   5.4  
At4g16820.1 68417.m02539 lipase class 3 family protein similar t...    27   7.1  

>At3g45300.1 68416.m04891 isovaleryl-CoA-dehydrogenase (IVD)
           identical to isovaleryl-CoA-dehydrogenase precursor
           [Arabidopsis thaliana] GI:5596622
          Length = 409

 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 40/113 (35%), Positives = 56/113 (49%), Gaps = 6/113 (5%)
 Frame = +2

Query: 272 DEQKALQELARKFTKDEIIPVAGQYDKTGEYPWPVVKKAWEV-GLMNGH---IPEHCGGL 439
           D Q   +E   KF +D I P A + DKT  +P  V    W++ G  N H    PE  GGL
Sbjct: 33  DTQLQFKESVSKFAQDNIAPHAERIDKTNSFPKDV--NLWKLMGEFNLHGITAPEEYGGL 90

Query: 440 GLGVFEACILSEELAYGCSGIMAAVY--ITEVGQTPIIIAGNKEQQKKYLGRL 592
           GLG    CI  EE++   SG +A  Y   + +    ++  G   Q++KYL +L
Sbjct: 91  GLGYLYHCIAMEEIS-RASGSVALSYGAHSNLCINQLVRNGTAAQKEKYLPKL 142


>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
            subunit (RPB205) (RPII) (RPB1) nearly identical to
            P|P18616 DNA-directed RNA polymerase II largest subunit
            (EC 2.7.7.6) {Arabidopsis thaliana}
          Length = 1840

 Score = 31.1 bits (67), Expect = 0.58
 Identities = 24/68 (35%), Positives = 29/68 (42%), Gaps = 1/68 (1%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSPV 352
            PTS  Y+    P +   S S     S +  P  P  S   P   PTS A+  T  GYSP 
Sbjct: 1652 PTSPAYSPTS-PSYSPTSPSYS-PTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPT 1709

Query: 351  L-SY*PAT 331
              SY P +
Sbjct: 1710 SPSYSPTS 1717



 Score = 28.7 bits (61), Expect = 3.1
 Identities = 23/68 (33%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSPV 352
            PTS  Y+    P +   S S     S +  P  P  S   P   PTS A+  T   YSP 
Sbjct: 1603 PTSPSYSPTS-PSYSPTSPSYS-PTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPT 1660

Query: 351  L-SY*PAT 331
              SY P +
Sbjct: 1661 SPSYSPTS 1668



 Score = 28.7 bits (61), Expect = 3.1
 Identities = 21/73 (28%), Positives = 31/73 (42%), Gaps = 2/73 (2%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTP--KPRPPQCSGMCPFIRPTSQAFFTTGHGYS 358
            PTS  Y+    P  P  S +    +  +P   P  P  S   P   PTS ++      YS
Sbjct: 1680 PTSPSYS----PTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYS 1735

Query: 357  PVLSY*PATGMIS 319
            P ++Y P+   +S
Sbjct: 1736 PSIAYSPSNARLS 1748



 Score = 27.9 bits (59), Expect = 5.4
 Identities = 20/59 (33%), Positives = 24/59 (40%)
 Frame = -1

Query: 531  PTSVMYTAAMIPEHP*ASSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSP 355
            PTS  Y+    P +   S S     S +  P  P  S   P   PTS A+  T   YSP
Sbjct: 1596 PTSPSYSPTS-PSYSPTSPSYS-PTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSP 1652


>At5g60090.1 68418.m07534 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 398

 Score = 27.9 bits (59), Expect = 5.4
 Identities = 12/23 (52%), Positives = 17/23 (73%), Gaps = 1/23 (4%)
 Frame = -3

Query: 529 NFGDVHRCHDTRASV-GQLLAQN 464
           NFG+VHRC+ +R +V G +  QN
Sbjct: 98  NFGEVHRCYFSRLNVDGAVKVQN 120


>At4g16820.1 68417.m02539 lipase class 3 family protein similar to
           DEFECTIVE IN ANTHER DEHISCENCE1 [Arabidopsis thaliana]
           GI:16215706; contains Pfam profile PF01764: Lipase
          Length = 517

 Score = 27.5 bits (58), Expect = 7.1
 Identities = 17/61 (27%), Positives = 28/61 (45%)
 Frame = -1

Query: 480 SSSLKIQASNTPKPRPPQCSGMCPFIRPTSQAFFTTGHGYSPVLSY*PATGMISSFVNLR 301
           S + +    + P+P+P Q     P +     + +TTG  ++P L+     G IS  V L 
Sbjct: 256 SENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLYTTGDQHAPSLAE-SLVGEISRLVELY 314

Query: 300 A 298
           A
Sbjct: 315 A 315


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,144,673
Number of Sequences: 28952
Number of extensions: 245652
Number of successful extensions: 774
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 574
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 759
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1180950720
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)